Tracing HIV-1 transmission: envelope traits of HIV-1 transmitter and recipient pairs.

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Serval ID
serval:BIB_F4E96DF335DA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tracing HIV-1 transmission: envelope traits of HIV-1 transmitter and recipient pairs.
Journal
Retrovirology
Author(s)
Oberle C.S., Joos B., Rusert P., Campbell N.K., Beauparlant D., Kuster H., Weber J., Schenkel C.D., Scherrer A.U., Magnus C., Kouyos R., Rieder P., Niederöst B., Braun D.L., Pavlovic J., Böni J., Yerly S., Klimkait T., Aubert V., Trkola A., Metzner K.J., Günthard H.F.
Working group(s)
Swiss HIV Cohort Study (SHCS)
Contributor(s)
Aubert V., Battegay M., Bernasconi E., Böni J., Braun D., Bucher H., Burton-Jeangros C., Calmy A., Cavassini M., Dollenmaier G., Egger M., Elzi L., Fehr J., Fellay J., Furrer H., Fux C., Gorgievski M., Günthard H., Haerry D., Hasse B., Hirsch H., Hoffmann M., Hösli I., Kahlert C., Kaiser L., Keiser O., Klimkait T., Kouyos R., Kovari H., Ledergerber B., Martinetti G., Martinez de Tejada B., Marzolini C., Metzner K., Müller N., Nadal D., Nicca D., Pantaleo G., Rauch A., Regenass S., Rudin C., Schöni-Affolter F., Schmid P., Speck R., Stöckle M., Tarr P., Trkola A., Vernazza P., Weber R., Yerly S.
ISSN
1742-4690 (Electronic)
ISSN-L
1742-4690
Publication state
Published
Issued date
05/09/2016
Peer-reviewed
Oui
Volume
13
Number
1
Pages
62
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Mucosal HIV-1 transmission predominantly results in a single transmitted/founder (T/F) virus establishing infection in the new host despite the generally high genetic diversity of the transmitter virus population. To what extent HIV-1 transmission is a stochastic process or driven by selective forces that allow T/F viruses best to overcome bottlenecks in transmission has not been conclusively resolved. Building on prior investigations that suggest HIV-1 envelope (Env) features to contribute in the selection process during transmission, we compared phenotypic virus characteristics of nine HIV-1 subtype B transmission pairs, six men who have sex with men and three male-to-female transmission pairs.
All recipients were identified early in acute infection and harbored based on extensive sequencing analysis a single T/F virus allowing a controlled analysis of virus properties in matched transmission pairs. Recipient and transmitter viruses from the closest time point to transmission showed no signs of selection for specific Env modifications such as variable loop length and glycosylation. Recipient viruses were resistant to circulating plasma antibodies of the transmitter and also showed no altered sensitivity to a large panel of entry inhibitors and neutralizing antibodies. The recipient virus did not consistently differ from the transmitter virus in terms of entry kinetics, cell-cell transmission and replicative capacity in primary cells. Our paired analysis revealed a higher sensitivity of several recipient virus isolates to interferon-α (IFNα) which suggests that resistance to IFNα cannot be a general driving force in T/F establishment.
With the exception of increased IFNα sensitivity, none of the phenotypic virus properties we investigated clearly distinguished T/F viruses from their matched transmitter viruses supporting the notion that at least in subtype B infection HIV-1 transmission is to a considerable extent stochastic.

Keywords
Acute Disease, Antibodies, Neutralizing/blood, Antibodies, Neutralizing/immunology, Female, Genetic Variation, HIV Infections/transmission, HIV Infections/virology, HIV-1/genetics, HIV-1/isolation & purification, HIV-1/physiology, Homosexuality, Male, Humans, Interferon-alpha/pharmacology, Leukocytes, Mononuclear/drug effects, Leukocytes, Mononuclear/virology, Male, Neutralization Tests, Phenotype, Sequence Analysis, DNA, Stochastic Processes, Viral Tropism, Virus Internalization, env Gene Products, Human Immunodeficiency Virus/genetics, env Gene Products, Human Immunodeficiency Virus/immunology
Pubmed
Open Access
Yes
Create date
09/09/2016 10:24
Last modification date
20/08/2019 16:21
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