Interaction of DNA with nuclear scaffolds in vitro

Details

Serval ID
serval:BIB_F4DB202D41B1
Type
Article: article from journal or magazin.
Collection
Publications
Title
Interaction of DNA with nuclear scaffolds in vitro
Journal
Journal of Molecular Biology
Author(s)
Izaurralde  E., Mirkovitch  J., Laemmli  U. K.
ISSN
0022-2836 (Print)
Publication state
Published
Issued date
03/1988
Volume
200
Number
1
Pages
111-25
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 5
Abstract
We have previously identified a number of specific DNA fragments called SARs (scaffold-associated regions) that are associated with the nuclear scaffold and define the basis of DNA loops. We demonstrate that cloned DNA fragments containing SAR sequences bind to nuclear scaffolds in vitro with the same specificity as have genomic SAR fragments. This specific interaction is observed with the biochemically complex type I scaffolds. These scaffolds are composed of the nuclear lamina proteins and a set of other proteins that forms the internal network of these structures. So-called type II scaffolds, which are composed primarily of the lamina proteins and lack the proteins of the internal network, do not bind the SAR fragments at a detectable level. Competition experiments show that different SARs share common structural elements and can bind to the same sites on the nuclear scaffold, although with different affinities. Moreover, the SAR binding sites appear to be evolutionarily conserved, as all the Drosophila SARs also bind with identical specificity to nuclear scaffolds derived from rat liver nuclei. These Sar interaction studies were carried out with lithium 3,5-diiodosalicylate-extracted nuclei. Interestingly, scaffolds prepared by high-salt extraction also bind the genomic and exogenously added SAR fragments specifically. However, the endogenous transcribed sequences, as opposed to the same fragments added as purified DNA, associate randomly with these scaffolds.
Keywords
Animals Binding Sites Chromosome Mapping DNA/*metabolism Drosophila melanogaster Evolution Nuclear Proteins/*metabolism Nucleic Acid Conformation
Pubmed
Web of science
Create date
25/01/2008 8:41
Last modification date
20/08/2019 16:21
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