UVR8-mediated inhibition of shade avoidance involves HFR1 stabilization in Arabidopsis.

Details

Serval ID
serval:BIB_F4BCB657A029
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
UVR8-mediated inhibition of shade avoidance involves HFR1 stabilization in Arabidopsis.
Journal
PLoS genetics
Author(s)
Tavridou E., Schmid-Siegert E., Fankhauser C., Ulm R.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Publication state
Published
Issued date
05/2020
Peer-reviewed
Oui
Volume
16
Number
5
Pages
e1008797
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Sun-loving plants perceive the proximity of potential light-competing neighboring plants as a reduction in the red:far-red ratio (R:FR), which elicits a suite of responses called the "shade avoidance syndrome" (SAS). Changes in R:FR are primarily perceived by phytochrome B (phyB), whereas UV-B perceived by UV RESISTANCE LOCUS 8 (UVR8) elicits opposing responses to provide a counterbalance to SAS, including reduced shade-induced hypocotyl and petiole elongation. Here we show at the genome-wide level that UVR8 broadly suppresses shade-induced gene expression. A subset of this gene regulation is dependent on the UVR8-stabilized atypical bHLH transcription regulator LONG HYPOCOTYL IN FAR-RED 1 (HFR1), which functions in part redundantly with PHYTOCHROME INTERACTING FACTOR 3-LIKE 1 (PIL1). In parallel, UVR8 signaling decreases protein levels of the key positive regulators of SAS, namely the bHLH transcription factors PHYTOCHROME INTERACTING FACTOR 4 (PIF4) and PIF5, in a COP1-dependent but HFR1-independent manner. We propose that UV-B antagonizes SAS via two mechanisms: degradation of PIF4 and PIF5, and HFR1- and PIL1-mediated inhibition of PIF4 and PIF5 function. This work highlights the importance of typical and atypical bHLH transcription regulators for the integration of light signals from different photoreceptors and provides further mechanistic insight into the crosstalk of UVR8 signaling and SAS.
Keywords
Arabidopsis/drug effects, Arabidopsis/genetics, Arabidopsis/metabolism, Arabidopsis Proteins/chemistry, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Basic Helix-Loop-Helix Transcription Factors/chemistry, Basic Helix-Loop-Helix Transcription Factors/genetics, Basic Helix-Loop-Helix Transcription Factors/metabolism, Chromatin/metabolism, Chromosomal Proteins, Non-Histone/genetics, Chromosomal Proteins, Non-Histone/metabolism, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Down-Regulation, Gene Expression Regulation, Plant/radiation effects, Protein Stability, Proteolysis, Transcription Factors/genetics, Transcription Factors/metabolism, Ubiquitin-Protein Ligases/genetics, Ubiquitin-Protein Ligases/metabolism, Ultraviolet Rays/adverse effects
Pubmed
Web of science
Open Access
Yes
Create date
15/06/2020 15:36
Last modification date
09/09/2020 6:26
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