An improved porcine model of stable methacholine-induced bronchospasm.

Details

Serval ID
serval:BIB_F4B60565325B
Type
Article: article from journal or magazin.
Collection
Publications
Title
An improved porcine model of stable methacholine-induced bronchospasm.
Journal
Intensive Care Medicine
Author(s)
Watremez C., Roeseler J., De Kock M., Clerbaux T., Detry B., Veriter C., Reynaert M., Gianello P., Jolliet P., Liistro G.
ISSN
0342-4642 (Print)
ISSN-L
0342-4642
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
29
Number
1
Pages
119-125
Language
english
Notes
Publication types: Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
OBJECTIVE: To validate an animal model replicating the pathophysiological characteristics of severe induced bronchospasm observed in humans, with a high level of stability permitting measurements such as the assessment of ventilation-perfusion relationships with the multiple inert gas elimination technique.
DESIGN AND SETTING: Experimental study in an animal research laboratory.
SUBJECTS: 13 piglets (age 3-4 months) were studied and 7 underwent the complete protocol
INTERVENTIONS: The animals were anesthetized and paralyzed. Mechanical ventilation was initiated in a volume-controlled mode. Ventilatory parameters were adjusted to obtain normocapnia and were maintained constant during the bronchospasm. Methacholine was administered via a synchronized nebulizer and progressively adjusted to obtain a stable twofold increase in peak inspiratory pressure.
MEASUREMENTS AND RESULTS: Cardiopulmonary physiological data including assessment of lung mechanics and measurement of ventilation-perfusion relationships were obtained before and during the bronchospasm. Peak inspiratory pressure increased from 19.7+/-2.9 to 44.4+/-7.1 cmH(2)O during the bronchospasm. The latter remained stable over 2 h. Respiratory mechanics, gas exchange, and ventilation-perfusion distribution changes typical of those observed in severe bronchospasm in humans were observed in all animals.
CONCLUSIONS: The present experimental model replicates some of the physiopathological characteristics of severe human bronchospasm, and its stability should facilitate studies of the effects of different ventilatory modes in the setting of acute severe asthma.
Keywords
Animals, Asthma/physiopathology, Bronchial Spasm/chemically induced, Bronchial Spasm/physiopathology, Disease Models, Animal, Methacholine Chloride/pharmacology, Reproducibility of Results, Respiration, Artificial, Respiratory Mechanics, Swine, Ventilation-Perfusion Ratio
Pubmed
Web of science
Create date
27/06/2013 14:41
Last modification date
20/08/2019 16:21
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