N-acetyl-L-cysteine exhibits antitumoral activity by increasing tumor necrosis factor alpha-dependent T-cell cytotoxicity

Details

Serval ID
serval:BIB_F3DD47AED881
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
N-acetyl-L-cysteine exhibits antitumoral activity by increasing tumor necrosis factor alpha-dependent T-cell cytotoxicity
Journal
Blood
Author(s)
Delneste  Y., Jeannin  P., Potier  L., Romero  P., Bonnefoy  J. Y.
ISSN
0006-4971 (Print)
Publication state
Published
Issued date
08/1997
Volume
90
Number
3
Pages
1124-32
Notes
Journal Article --- Old month value: Aug 1
Abstract
Because of its anticarcinogenic and antimutagenic properties, N-acetyl-L-cysteine (NAC) has been proposed for cancer treatment. Here we present a mechanism of action for NAC in cancer. Our data show that NAC (1) induces an early and sustained increase of membrane tumor necrosis factor alpha (TNF alpha) expression on human stimulated-peripheral blood (PB) T cells and (2) increases membrane TNF-RI and TNF-RII on tumoral cell lines and on T cells after stimulation. These effects result from an early inhibition of both TNF alpha and TNF-R shedding, as well as a later increase of the respective mRNA expression. Consequently, NAC confers cytotoxic properties to human PB T cells through a membrane TNF alpha-dependent pathway. In vivo, NAC given orally inhibits tumor appearance in more than a third (18 out of 50) B6D2F1 mice injected with L1210 lymphoma cells. Spleen cells from protected mice killed L1210 lymphoma cells in vitro in a membrane TNF alpha-dependent manner. Furthermore these mice were resistant to a second inoculation of L1210 cells without further treatment with NAC. Thus, NAC exhibits a potent antitumoral activity by modulating TNF alpha and TNF-R processing without showing any in vitro and in vivo toxicity.
Keywords
Acetylcysteine/*pharmacology/therapeutic use Adjuvants, Immunologic/*pharmacology/therapeutic use Animals Antineoplastic Agents/*pharmacology/therapeutic use Cells, Cultured Cytotoxicity, Immunologic/drug effects Gene Expression Regulation/drug effects Gene Expression Regulation, Leukemic/drug effects Humans Leukemia L1210/drug therapy/immunology/pathology Mice Mice, Inbred BALB C Neoplasm Proteins/biosynthesis/genetics Neoplasm Transplantation Receptors, Tumor Necrosis Factor/biosynthesis/genetics T-Lymphocytes, Cytotoxic/*drug effects/immunology Tumor Necrosis Factor-alpha/biosynthesis/genetics/*physiology
Pubmed
Web of science
Create date
28/01/2008 11:28
Last modification date
20/08/2019 16:20
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