Expression analysis of genes involved in DNA repair or synthesis in mixed neuroendocrine/nonneuroendocrine carcinomas.

Details

Serval ID
serval:BIB_F3C57FEEAAE9
Type
Article: article from journal or magazin.
Collection
Publications
Title
Expression analysis of genes involved in DNA repair or synthesis in mixed neuroendocrine/nonneuroendocrine carcinomas.
Journal
Neuroendocrinology
Author(s)
Volante M., Monica V., Birocco N., Brizzi M.P., Busso S., Daniele L., La Rosa S., Righi L., Sapino A., Berruti A., Scagliotti G.V., Papotti M.
ISSN
1423-0194 (Electronic)
ISSN-L
0028-3835
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
101
Number
2
Pages
151-160
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
BACKGROUND: Mixed neuroendocrine/nonneuroendocrine carcinomas are heterogeneous tumors with poorly defined diagnostic and clinical features and without pathological or molecular markers of prognosis or markers predicting their response to therapy. We aimed at analyzing the pathological features and the expression of genes involved in DNA repair or synthesis in a cohort of patients with mixed carcinomas from different sites as compared to the patients' outcome.
METHODS: Relative cDNA quantification of ribonucleotide reductase, large subunit 1, excision repair cross-complementation group 1, thymidylate synthase and topoisomerase IIa genes was tested using real-time PCR on microdissected neuroendocrine and nonneuroendocrine tumor components of 42 mixed cases (from the lung as well as the gastrointestinal and genitourinary tracts) and on 45 control cases of pure neuroendocrine and nonneuroendocrine carcinomas.
RESULTS: The expression levels of all genes were stable comparing nonneuroendocrine and neuroendocrine components of mixed cases (except for topoisomerase IIa in lung samples) but significantly different as compared to control nonneuroendocrine and neuroendocrine tumors. In the multivariate analysis including all clinical and pathological parameters and gene expression levels available, a predominant nonneuroendocrine component, the administration of additional therapy other than surgery and a high thymidylate synthase expression in nonneuroendocrine tumor tissue were significantly associated with a lower risk of a patient's death.
CONCLUSIONS: Our data show that mixed neuroendocrine/nonneuroendocrine carcinomas are different at the molecular level from their pure neuroendocrine and nonneuroendocrine counterparts, and detailed analyses of their clinical, pathological and molecular features may improve the clinical strategies for the treatment of these rare and underestimated tumors.
Keywords
Aged, Aged, 80 and over, Carcinoma/genetics, Carcinoma/metabolism, Carcinoma, Neuroendocrine/genetics, Carcinoma, Neuroendocrine/metabolism, DNA Repair/genetics, Female, Follow-Up Studies, Gastrointestinal Neoplasms/genetics, Gastrointestinal Neoplasms/metabolism, Gene Expression Regulation, Neoplastic, Humans, Male, Middle Aged, Multivariate Analysis, Survival Analysis, Urogenital Neoplasms/genetics, Urogenital Neoplasms/metabolism
Pubmed
Web of science
Create date
06/09/2016 11:54
Last modification date
20/08/2019 16:20
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