Analysis of the mouse Scnn1a promoter in cortical collecting duct cells and in transgenic mice

Details

Serval ID
serval:BIB_F35C8F3D41CC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of the mouse Scnn1a promoter in cortical collecting duct cells and in transgenic mice
Journal
Biochimica et Biophysica Acta-Gene Structure and Expression
Author(s)
Kohler S., Pradervand S., Verdumo C., Mérillat A. M., Bens M., Vandewalle A., Beermann F., Hummler E.
ISSN
0167-4781
ISSN-L
1879-2634
Publication state
Published
Issued date
05/2001
Peer-reviewed
Oui
Volume
1519
Number
1-2
Pages
106-10
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: May 28
Abstract
We have isolated and characterised the promoter of the mouse Scnn1a (alpha ENaC) gene. Using transient transfections of serial deletion mutants into Scnn1a-expressing cells, we demonstrate that 1.56 kb of 5' upstream sequence is required for cell-specific expression and corticosteroid-mediated regulation. These 5' sequences are not sufficient to drive expression of a lacZ reporter gene or a rat Scnn1a cDNA in transgenic mice, where they failed to rescue Scnn1a deficiency.
Keywords
Amino Acid Sequence Animals Base Sequence Cells, Cultured Consensus Sequence Epithelial Sodium Channel Gene Expression Regulation Kidney Cortex/*metabolism Kidney Tubules, Collecting/*metabolism Mice Mice, Transgenic Molecular Sequence Data *Promoter Regions (Genetics) Sodium Channels/*genetics Transfection
Pubmed
Web of science
Create date
24/01/2008 12:42
Last modification date
20/08/2019 16:20
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