Oxidation proteomics: the role of thiol modifications

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State: Public
Version: author
Serval ID
serval:BIB_F23E1D0CEB06
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Oxidation proteomics: the role of thiol modifications
Journal
Current Proteomics
Author(s)
Riederer B.M
ISSN
1570-1646
Publication state
Published
Issued date
2009
Volume
6
Number
1
Pages
51-62
Language
english
Abstract
Identification of thiol modifications has gained significant importance. It is increasingly recognized that cysteines play an important role in protein function under both physiological and patho-physiological conditions. Here we reviewed different approaches that are used to identify oxidized proteins and discuss different fluorescent labeling techniques, differential two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization - time of flight identification, in short MALDI-TOF. We illuminate processes that depend on protein oxidation of cysteines and we look into consequences of thiol oxidation during aging and in a variety of diseases, with a special reference to Alzheimer's disease. There is an urgent need for methods that detect specifically oxidized proteins and are able to distinguish different oxidation types.
Keywords
Aging , cysteine , oxidation , thiol , DIGE , MALDI-TOF , Western blot , dye labeling , disease
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Create date
23/08/2010 14:24
Last modification date
20/08/2019 17:19
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