Olfactory receptor pseudo-pseudogenes.

Details

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State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_F2281CE13F6D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Olfactory receptor pseudo-pseudogenes.
Journal
Nature
Author(s)
Prieto-Godino L.L., Rytz R., Bargeton B., Abuin L., Arguello J.R., Peraro M.D., Benton R.
ISSN
1476-4687 (Electronic)
ISSN-L
0028-0836
Publication state
Published
Issued date
03/11/2016
Peer-reviewed
Oui
Volume
539
Number
7627
Pages
93-97
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Pseudogenes are generally considered to be non-functional DNA sequences that arise through nonsense or frame-shift mutations of protein-coding genes. Although certain pseudogene-derived RNAs have regulatory roles, and some pseudogene fragments are translated, no clear functions for pseudogene-derived proteins are known. Olfactory receptor families contain many pseudogenes, which reflect low selection pressures on loci no longer relevant to the fitness of a species. Here we report the characterization of a pseudogene in the chemosensory variant ionotropic glutamate receptor repertoire of Drosophila sechellia, an insect endemic to the Seychelles that feeds almost exclusively on the ripe fruit of Morinda citrifolia. This locus, D. sechellia Ir75a, bears a premature termination codon (PTC) that appears to be fixed in the population. However, D. sechellia Ir75a encodes a functional receptor, owing to efficient translational read-through of the PTC. Read-through is detected only in neurons and is independent of the type of termination codon, but depends on the sequence downstream of the PTC. Furthermore, although the intact Drosophila melanogaster Ir75a orthologue detects acetic acid-a chemical cue important for locating fermenting food found only at trace levels in Morinda fruit-D. sechellia Ir75a has evolved distinct odour-tuning properties through amino-acid changes in its ligand-binding domain. We identify functional PTC-containing loci within different olfactory receptor repertoires and species, suggesting that such 'pseudo-pseudogenes' could represent a widespread phenomenon.

Keywords
Acetic Acid/metabolism, Animals, Base Sequence, Codon, Terminator/genetics, Drosophila/genetics, Drosophila/metabolism, Drosophila melanogaster/genetics, Drosophila melanogaster/metabolism, Ligands, Molecular Sequence Annotation, Neurons/metabolism, Organ Specificity, Peptide Chain Elongation, Translational, Pseudogenes/genetics, Receptors, Odorant/biosynthesis, Receptors, Odorant/genetics, Receptors, Odorant/metabolism, Reproducibility of Results
Pubmed
Create date
06/09/2016 13:34
Last modification date
20/08/2019 17:19
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