NLRP12 is a neutrophil-specific, negative regulator of in vitro cell migration but does not modulate LPS- or infection-induced NF-κB or ERK signalling.

Details

Serval ID
serval:BIB_F156CAAFEF5C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
NLRP12 is a neutrophil-specific, negative regulator of in vitro cell migration but does not modulate LPS- or infection-induced NF-κB or ERK signalling.
Journal
Immunobiology
Author(s)
Zamoshnikova A., Groß C.J., Schuster S., Chen K.W., Wilson A., Tacchini-Cottier F., Schroder K.
ISSN
1878-3279 (Electronic)
ISSN-L
0171-2985
Publication state
Published
Issued date
2016
Volume
221
Number
2
Pages
341-346
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
NOD-like receptors (NLR) are a family of cytosolic pattern recognition receptors that include many key drivers of innate immune responses. NLRP12 is an emerging member of the NLR family that is closely related to the well-known inflammasome scaffold, NLRP3. Since its discovery, various functions have been proposed for NLRP12, including the positive regulation of dendritic cell (DC) and neutrophil migration and the inhibition of NF-κB and ERK signalling in DC and macrophages. We show here that NLRP12 is poorly expressed in murine macrophages and DC, but is strongly expressed in neutrophils. Using myeloid cells from WT and Nlrp12(-/)(-) mice, we show that, contrary to previous reports, NLRP12 does not suppress LPS- or infection-induced NF-κB or ERK activation in myeloid cells, and is not required for DC migration in vitro. Surprisingly, we found that Nlrp12 deficiency caused increased rather than decreased neutrophil migration towards the chemokine CXCL1 and the neutrophil parasite Leishmania major, revealing NLRP12 as a negative regulator of directed neutrophil migration under these conditions.
Keywords
Animals, Cell Movement/drug effects, Cell Movement/immunology, Chemokine CXCL1/genetics, Chemokine CXCL1/immunology, Dendritic Cells/immunology, Dendritic Cells/parasitology, Female, Gene Expression Regulation, Intracellular Signaling Peptides and Proteins/deficiency, Intracellular Signaling Peptides and Proteins/genetics, Leishmania major/immunology, Lipopolysaccharides/pharmacology, Macrophages/immunology, Macrophages/parasitology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mitogen-Activated Protein Kinase 1/genetics, Mitogen-Activated Protein Kinase 1/immunology, Mitogen-Activated Protein Kinase 3/genetics, Mitogen-Activated Protein Kinase 3/immunology, NF-kappa B/genetics, NF-kappa B/immunology, Neutrophils/immunology, Neutrophils/parasitology, Organ Specificity, Signal Transduction
Pubmed
Web of science
Create date
19/01/2016 12:16
Last modification date
20/08/2019 17:18
Usage data