Multiscattering-enhanced absorbance to enable the sensitive analysis of extremely diluted biological samples: Determination of oxidative potential in exhaled air

Details

Serval ID
serval:BIB_F1115EB2E5C5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Multiscattering-enhanced absorbance to enable the sensitive analysis of extremely diluted biological samples: Determination of oxidative potential in exhaled air
Journal
Medicine in Novel Technology and Devices
Author(s)
Goekce Sami, Concha-Lozano Nicolas, Sauvain Jean-Jacques, Hemmendinger Maud, Portela Alejandro, Sergent Elise, Andujar Pascal, Pairon Jean-Claude, Wild Pascal, Suárez Guillaume
ISSN
2590-0935
Publication state
Published
Issued date
06/2022
Volume
14
Pages
100120
Language
english
Notes
L2017000611
2022-03-01
Abstract
Direct determination of the oxidative potential in exhaled air (OPEA) as a biological indicator of the redox balance status in airways requires an extremely sensitive sensing approach to counterbalance the high dilution of the exhaled air matrix. In opposite to standard colorimetric assays based on Beer-Lambert Law, the use of simple multiscattering-enhanced absorbance (MEA) strategy results in an improved photonic sensing system. Based on this strategy and using the ferrous-xylenol orange assay, analytical performances allowing the detection of 3 ​pmol of H2O2 could be obtained. The comparative analysis of photometric configurations – standard vs MEA – highlights the large absorbance gain obtained for tiny H2O2 amounts – from 0 to 30 ​pmol – whereas similar sensing trends are observed at higher concentration range. The automated introduction of exhaled air sample into the reaction compartment via needle-impinger coupled with the MEA-based photonic cell enables ultrasensitive determination of OPEA in short time (<5 ​min). A pilot study conducted on healthy subjects (n ​= ​22) and chronic obstructive pulmonary disease (COPD) patients (n ​= ​23) volunteers shows a significant increase of the OPEA for COPD versus controls and strong negative correlation between OPEA and spirometry parameters (Tiffeneau index: FEV1/FVC) for ex-/non-smokers. Application perspectives to assess inflammatory episodes in obstructive lung diseases are thus made possible using this sensing approach.
Keywords
Computer Science Applications, Biomedical Engineering, Medicine (miscellaneous)
Open Access
Yes
Funding(s)
Swiss National Science Foundation
Create date
04/03/2022 10:23
Last modification date
15/03/2022 7:34
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