Identification of the 33-kDa alkaline protease of Aspergillus fumigatus in vitro and in vivo

Details

Serval ID
serval:BIB_F0B3DCC06E10
Type
Article: article from journal or magazin.
Collection
Publications
Title
Identification of the 33-kDa alkaline protease of Aspergillus fumigatus in vitro and in vivo
Journal
Journal of Medical Microbiology
Author(s)
Moutaouakil  M., Monod  M., Prevost  M. C., Bouchara  J. P., Paris  S., Latge  J. P.
ISSN
0022-2615 (Print)
Publication state
Published
Issued date
11/1993
Volume
39
Number
5
Pages
393-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
Aspergillus fumigatus produced a 33-kDa serine protease (ALP) in vitro and in vivo. In vitro, this alkaline protease was secreted when the fungus was cultivated in the absence of protein, if the pH of the medium remained close to neutrality. Western blotting and immunoelectronmicroscopy studies showed that ALP was localised in the wall of the fungus and was degraded after secretion in the culture medium under conditions of low pH. Although present in the lung during infection, ALP did not appear to be diagnostically useful and was different from the precipitating chymotrypsin antigen used in the diagnosis of aspergilloma.
Keywords
Animals Aspergillosis/enzymology/*microbiology Aspergillus fumigatus/*enzymology Blotting, Western Culture Media Fluorescent Antibody Technique Humans Hydrogen-Ion Concentration Immune Sera/immunology Immunoblotting Lung/enzymology/microbiology Male Mice Rabbits Serine Endopeptidases/*biosynthesis/immunology/isolation & purification
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 17:46
Last modification date
09/05/2019 3:16
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