Identification of novel secreted proteases during extracellular proteolysis by dermatophytes at acidic pH.

Details

Serval ID
serval:BIB_F007F4C7B959
Type
Article: article from journal or magazin.
Collection
Publications
Title
Identification of novel secreted proteases during extracellular proteolysis by dermatophytes at acidic pH.
Journal
Proteomics
Author(s)
Sriranganadane D., Waridel P., Salamin K., Feuermann M., Mignon B., Staib P., Neuhaus J.M., Quadroni M., Monod M.
ISSN
1615-9861 (Electronic)
ISSN-L
1615-9853
Publication state
Published
Issued date
2011
Volume
11
Number
22
Pages
4422-4433
Language
english
Abstract
The dermatophytes are a group of closely related fungi which are responsible for the great majority of superficial mycoses in humans and animals. Among various potential virulence factors, their secreted proteolytic activity attracts a lot of attention. Most dermatophyte-secreted proteases which have so far been isolated in vitro are neutral or alkaline enzymes. However, inspection of the recently decoded dermatophyte genomes revealed many other hypothetical secreted proteases, in particular acidic proteases similar to those characterized in Aspergillus spp. The validation of such genome predictions instigated the present study on two dermatophyte species, Microsporum canis and Arthroderma benhamiae. Both fungi were found to grow well in a protein medium at acidic pH, accompanied by extracellular proteolysis. Shotgun MS analysis of secreted protein revealed fundamentally different protease profiles during fungal growth in acidic versus neutral pH conditions. Most notably, novel dermatophyte-secreted proteases were identified at acidic pH such as pepsins, sedolisins and acidic carboxypeptidases. Therefore, our results not only support genome predictions, but demonstrate for the first time the secretion of acidic proteases by dermatophytes. Our findings also suggest the existence of different pathways of protein degradation into amino acids and short peptides in these highly specialized pathogenic fungi.
Pubmed
Web of science
Create date
05/01/2012 16:46
Last modification date
20/08/2019 16:17
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