CD32<sup>+</sup> and PD-1<sup>+</sup> Lymph Node CD4 T Cells Support Persistent HIV-1 Transcription in Treated Aviremic Individuals.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_EFF772664CD3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
CD32<sup>+</sup> and PD-1<sup>+</sup> Lymph Node CD4 T Cells Support Persistent HIV-1 Transcription in Treated Aviremic Individuals.
Journal
Journal of virology
Author(s)
Noto A., Procopio F.A., Banga R., Suffiotti M., Corpataux J.M., Cavassini M., Riva A., Fenwick C., Gottardo R., Perreau M., Pantaleo G.
ISSN
1098-5514 (Electronic)
ISSN-L
0022-538X
Publication state
Published
Issued date
15/10/2018
Peer-reviewed
Oui
Volume
92
Number
20
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
A recent study conducted in blood has proposed CD32 as the marker identifying the "elusive" HIV reservoir. We have investigated the distribution of CD32 <sup>+</sup> CD4 T cells in blood and lymph nodes (LNs) of HIV-1-uninfected subjects and viremic untreated and long-term-treated HIV-1-infected individuals and their relationship with PD-1 <sup>+</sup> CD4 T cells. The frequency of CD32 <sup>+</sup> CD4 T cells was increased in viremic compared to treated individuals in LNs, and a large proportion (up to 50%) of CD32 <sup>+</sup> cells coexpressed PD-1 and were enriched within T follicular helper (Tfh) cells. We next investigated the role of LN CD32 <sup>+</sup> CD4 T cells in the HIV reservoir. Total HIV DNA was enriched in CD32 <sup>+</sup> and PD-1 <sup>+</sup> CD4 T cells compared to CD32 <sup>-</sup> and PD-1 <sup>-</sup> cells in both viremic and treated individuals, but there was no difference between CD32 <sup>+</sup> and PD-1 <sup>+</sup> cells. There was no enrichment of latently infected cells with inducible HIV-1 in CD32 <sup>+</sup> versus PD-1 <sup>+</sup> cells in antiretroviral therapy (ART)-treated individuals. HIV-1 transcription was then analyzed in LN memory CD4 T cell populations sorted on the basis of CD32 and PD-1 expression. CD32 <sup>+</sup> PD-1 <sup>+</sup> CD4 T cells were significantly enriched in cell-associated HIV RNA compared to CD32 <sup>-</sup> PD-1 <sup>-</sup> (averages of 5.2-fold in treated individuals and 86.6-fold in viremics), CD32 <sup>+</sup> PD-1 <sup>-</sup> (2.2-fold in treated individuals and 4.3-fold in viremics), and CD32 <sup>-</sup> PD-1 <sup>+</sup> (2.2-fold in ART-treated individuals and 4.6-fold in viremics) cell populations. Similar levels of HIV-1 transcription were found in CD32 <sup>+</sup> PD-1 <sup>-</sup> and CD32 <sup>-</sup> PD-1 <sup>+</sup> CD4 T cells. Interestingly, the proportion of CD32 <sup>+</sup> and PD-1 <sup>+</sup> CD4 T cells negatively correlated with CD4 T cell counts and length of therapy. Therefore, the expression of CD32 identifies, independently of PD-1, a CD4 T cell population with persistent HIV-1 transcription and coexpression of CD32 and PD-1, the CD4 T cell population with the highest levels of HIV-1 transcription in both viremic and treated individuals.IMPORTANCE The existence of long-lived latently infected resting memory CD4 T cells represents a major obstacle to the eradication of HIV infection. Identifying cell markers defining latently infected cells containing replication-competent virus is important in order to determine the mechanisms of HIV persistence and to develop novel therapeutic strategies to cure HIV infection. We provide evidence that PD-1 and CD32 may have a complementary role in better defining CD4 T cell populations infected with HIV-1. Furthermore, CD4 T cells coexpressing CD32 and PD-1 identify a CD4 T cell population with high levels of persistent HIV-1 transcription.
Keywords
Adult, Anti-Retroviral Agents/therapeutic use, CD4-Positive T-Lymphocytes/chemistry, CD4-Positive T-Lymphocytes/virology, DNA, Viral/analysis, Female, HIV Infections/drug therapy, HIV Infections/pathology, HIV-1/growth & development, Humans, Lymph Nodes/pathology, Male, Middle Aged, Programmed Cell Death 1 Receptor/analysis, RNA, Viral/analysis, Receptors, IgG/analysis, T-Lymphocyte Subsets/chemistry, T-Lymphocyte Subsets/virology, Transcription, Genetic, Young Adult, CD32, PD-1, Tfh cells, human immunodeficiency virus, lymph node
Pubmed
Web of science
Open Access
Yes
Create date
18/07/2018 16:22
Last modification date
20/08/2019 17:17
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