Mouse Model of Respiratory Tract Infection Induced by Waddlia chondrophila.

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Version: Final published version
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Serval ID
serval:BIB_EF5484090AE6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mouse Model of Respiratory Tract Infection Induced by Waddlia chondrophila.
Journal
Plos One
Author(s)
Pilloux L., LeRoy D., Brunel C., Roger T. (co-last), Greub G. (co-last)
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
11
Number
3
Pages
e0150909
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Waddlia chondrophila, an obligate intracellular bacterium belonging to the Chlamydiales order, is considered as an emerging pathogen. Some clinical studies highlighted a possible role of W. chondrophila in bronchiolitis, pneumonia and miscarriage. This pathogenic potential is further supported by the ability of W. chondrophila to infect and replicate within human pneumocytes, macrophages and endometrial cells. Considering that W. chondrophila might be a causative agent of respiratory tract infection, we developed a mouse model of respiratory tract infection to get insight into the pathogenesis of W. chondrophila. Following intranasal inoculation of 2 x 108 W. chondrophila, mice lost up to 40% of their body weight, and succumbed rapidly from infection with a death rate reaching 50% at day 4 post-inoculation. Bacterial loads, estimated by qPCR, increased from day 0 to day 3 post-infection and decreased thereafter in surviving mice. Bacterial growth was confirmed by detecting dividing bacteria using electron microscopy, and living bacteria were isolated from lungs 14 days post-infection. Immunohistochemistry and histopathology of infected lungs revealed the presence of bacteria associated with pneumonia characterized by an important multifocal inflammation. The high inflammatory score in the lungs was associated with the presence of pro-inflammatory cytokines in both serum and lungs at day 3 post-infection. This animal model supports the role of W. chondrophila as an agent of respiratory tract infection, and will help understanding the pathogenesis of this strict intracellular bacterium.
Pubmed
Web of science
Open Access
Yes
Create date
10/03/2016 19:16
Last modification date
09/04/2021 6:35
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