Paradoxical attenuation of leukocyte rolling in response to ischemia- reperfusion and extracorporeal blood circulation in inflamed tissue

Details

Serval ID
serval:BIB_EF3BE583255B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Paradoxical attenuation of leukocyte rolling in response to ischemia- reperfusion and extracorporeal blood circulation in inflamed tissue
Journal
American Journal of Physiology - Heart and Circulatory Physiology
Author(s)
Schafer  S. C., Sehrt  D. N., Kamler  M., Jakob  H., Lehr  H. A.
ISSN
0363-6135 (Print)
Publication state
Published
Issued date
2005
Volume
289
Number
1
Pages
H330-H335
Notes
PT - Journal Article PT - Validation Studies
Abstract
In contrast to acute preparations such as the exteriorized mesentery or the cremaster muscle, chronically instrumented chamber models allow one to study the microcirculation under "physiological" conditions, i.e., in the absence of trauma-induced leukocyte rolling along the venular endothelium. To underscore the importance of studying the naive microcirculation, we implanted titanium dorsal skinfold chambers in hamsters and used intravital fluorescence microscopy to study venular leukocyte rolling in response to ischemia-reperfusion injury or extracorporeal blood circulation. The experiments were performed in chambers that fulfilled all well-established criteria for a physiological microcirculation as well as in chambers that showed various extents of leukocyte rolling due to trauma, hemorrhage, or inflammation. In ideal chambers with a physiological microcirculation (<30 rolling leukocytes/mm vessel circumference in 30 s), ischemia-reperfusion injury and extracorporeal blood circulation significantly stimulated leukocyte rolling along the venular endothelium and, subsequently, firm leukocyte adhesion. In contrast, both stimuli failed to elicit leukocyte rolling in borderline chambers (30-100 leukocytes/mm), and in blatantly inflamed chambers with yet higher numbers of rolling leukocytes at baseline (>100 leukocytes/mm), we observed a paradoxical reduction of leukocyte rolling after ischemia-reperfusion injury or extracorporeal blood circulation. A similar effect was observed when we superfused leukotriene B4 (LTB4) onto the chamber tissue. The initial increase in leukocyte rolling in response to an LTB4 challenge was reversed by a second superfusion 90 min later. These observations underscore 1) the benefit of studying leukocyte-endothelial cell interaction in chronically instrumented chamber models and 2) the necessity to strictly adhere to well-established criteria of a physiological microcirculation
Keywords
Animals/Cricetinae/Extracorporeal Circulation/Leukocyte Rolling/drug effects/Leukotriene B4/pharmacology/Mesocricetus/Microscopy,Fluorescence/Muscle,Skeletal/blood supply/Myositis/physiopathology/Reperfusion Injury/Venules
Pubmed
Web of science
Create date
29/01/2008 18:36
Last modification date
20/08/2019 16:16
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