A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.

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Serval ID
serval:BIB_EF00B2AF2B7D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.
Journal
Cell metabolism
Author(s)
Jourdain Alexis
Publication state
Published
Issued date
22/09/2016
Language
english
Abstract
Oxidative phosphorylation (OXPHOS) is the major pathway for ATP production in humans. Deficiencies in OXPHOS can arise from mutations in either mitochondrial or nuclear genomes and comprise the largest collection of inborn errors of metabolism. At present we lack a complete catalog of human genes and pathways essential for OXPHOS. Here we introduce a genome-wide CRISPR "death screen" that actively selects dying cells to reveal human genes required for OXPHOS, inspired by the classic observation that human cells deficient in OXPHOS survive in glucose but die in galactose. We report 191 high-confidence hits essential for OXPHOS, including 72 underlying known OXPHOS diseases. Our screen reveals a functional module consisting of NGRN, WBSCR16, RPUSD3, RPUSD4, TRUB2, and FASTKD2 that regulates the mitochondrial 16S rRNA and intra-mitochondrial translation. Our work yields a rich catalog of genes required for OXPHOS and, more generally, demonstrates the power of death screening for functional genomic analysis.
Pubmed
Create date
13/04/2021 17:25
Last modification date
14/04/2021 6:37
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