IL-4-dependent regulation of TGF-alpha and TGF-beta1 expression in human eosinophils
Details
Serval ID
serval:BIB_EE5F4DFF0263
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
IL-4-dependent regulation of TGF-alpha and TGF-beta1 expression in human eosinophils
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
06/1998
Volume
160
Number
12
Pages
6121-7
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun 15
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun 15
Abstract
TGFs play important roles in wound healing and carcinogenesis. We have previously demonstrated that eosinophils infiltrating into different pathologic processes elaborate TGF-alpha and TGF-beta1. Eosinophils infiltrating hamster cutaneous wounds were found to express TGFs sequentially. In this study, we examined the biologic mediators that may regulate the expression of TGF-alpha and -beta1 by eosinophils. Eosinophils were isolated from the peripheral blood of healthy donors and cultured in the absence or presence of IL-3, IL-4, and IL-5. Cells were analyzed by in situ hybridization and immunohistochemistry. Supernatants from these cultures were assayed for secreted TGF-alpha and TGF-beta1 using TGF-specific ELISAs. IL-3, IL-4, and IL-5 independently up-regulated TGF-beta1 mRNA and product expression by eosinophils in all donors. Interestingly, TGF-alpha production by eosinophils was up-regulated by IL-3 and IL-5 but was down-regulated by IL-4. Consistent with the ability of IL-4 to regulate eosinophil responses, IL-4 signaling molecules are present in human eosinophils. The observation that IL-4 can differentially regulate the expression of TGF-alpha and TGF-beta1 suggests that IL-4 may serve as a physiologic molecular switch of TGF expression by the infiltrating eosinophils in wound healing and carcinogenesis.
Keywords
Adult
Animals
Cricetinae
Eosinophils/drug effects/*metabolism
*Gene Expression Regulation/drug effects
Humans
Interleukin-3/pharmacology
Interleukin-4/pharmacology/*physiology
Interleukin-5/pharmacology
Protein Sorting Signals/metabolism
RNA, Messenger
STAT6 Transcription Factor
Trans-Activators/metabolism
Transforming Growth Factor alpha/*biosynthesis
Transforming Growth Factor beta/*biosynthesis
Pubmed
Web of science
Create date
25/01/2008 10:52
Last modification date
20/08/2019 17:15