Supramaximal Intensity Hypoxic Exercise and Vascular Function Assessment in Mice.

Details

Serval ID
serval:BIB_ED664A1697B9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Supramaximal Intensity Hypoxic Exercise and Vascular Function Assessment in Mice.
Journal
Journal of visualized experiments
Author(s)
Lavier J., Beaumann M., Ménetrey S., Mazzolai L., Peyter A.C., Pellegrin M., Millet G.P.
ISSN
1940-087X (Electronic)
ISSN-L
1940-087X
Publication state
Published
Issued date
15/03/2019
Peer-reviewed
Oui
Number
145
Language
english
Notes
Publication types: Journal Article ; Video-Audio Media
Publication Status: epublish
Abstract
Exercise training is an important strategy for maintaining health and preventing many chronic diseases. It is the first line of treatment recommended by international guidelines for patients suffering from cardiovascular diseases, more specifically, lower extremity artery diseases, where the patients' walking capacity is considerably altered, affecting their quality of life. Traditionally, both low continuous exercise and interval training have been used. Recently, supramaximal training has also been shown to improve athletes' performances via vascular adaptations, amongst other mechanisms. The combination of this type of training with hypoxia could bring an additional and/or synergic effect, which could be of interest for certain pathologies. Here, we describe how to perform supramaximal intensity training sessions in hypoxia on healthy mice at 150% of their maximal speed, using a motorized treadmill and a hypoxic box. We also show how to dissect the mouse in order to retrieve organs of interest, particularly the pulmonary artery, the abdominal aorta, and the iliac artery. Finally, we show how to perform ex vivo vascular function assessment on the retrieved vessels, using isometric tension studies.
Pubmed
Web of science
Create date
15/04/2019 9:43
Last modification date
21/08/2019 6:32
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