Measurement of plasma endothelin-1 in experimental hypertension and in healthy subjects.

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Serval ID
serval:BIB_ED3D531B181B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Measurement of plasma endothelin-1 in experimental hypertension and in healthy subjects.
Journal
American journal of hypertension
Author(s)
Abdel-Sayed S., Nussberger J., Aubert J.F., Gohlke P., Brunner H.R., Brakch N.
ISSN
0895-7061
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
16
Number
7
Pages
515-21
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
BACKGROUND: Endothelin-1 is an endothelium-derived potent vasoconstrictor peptide of 21 amino acids. To establish reference values in different models of hypertension and in human subjects an assay for plasma immunoreactive endothelin-1 (ET-1) was optimized. METHODS: ET-1 is extracted by acetone from 1 mL of plasma and subjected to a sensitive enzyme-linked immunosorbent assay. RESULTS: The detection limit for plasma ET-1 is 0.05 fmol/mL. Mean recoveries of the 1, 2, 5, and 10 fmol of ET-1 added to 1 mL of plasma were 66%, 75%, 85%, and 92%, respectively. Within- and between-assay coefficients of variation were < or =12% and < or =10%, respectively. Assay accuracy was demonstrated by consistent recoveries of added ET-1 over the entire physiologic range of plasma concentrations and by the linearity of ET-1 concentrations measured in serially diluted plasma extracts (r = 0.99). No ET-1 was detected when albumin buffer was extracted instead of plasma. Using this method, we found increased ET-1 levels in plasma of three experimental rat models of hypertension: stroke prone spontaneously hypertensive rats (SP-SHR), deoxycorticosterone acetate-salt hypertensive rats, and one kidney-one clip hypertensive rats. In contrast, plasma ET-1 levels of SHR were half those of normotensive Wistar rats. In two kidney-one clip hypertensive rats, plasma ET-1 concentrations were not different from those found in sham-operated control rats. Plasma ET-1 concentrations of 37 healthy men were 0.85 +/- 0.26 fmol/ml (mean +/- SD). CONCLUSIONS: The present assay reliably measures ET-1 levels in rat and human plasma. It allows to discriminate between different forms of hypertension with high or low circulating levels of ET-1.
Keywords
Animals, Endothelin-1, Enzyme-Linked Immunosorbent Assay, Humans, Hypertension, Male, Models, Animal, Rats, Rats, Wistar
Pubmed
Web of science
Open Access
Yes
Create date
05/03/2008 17:40
Last modification date
25/09/2019 7:11
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