Use of long synthetic peptides to study the antigenicity and immunogenicity of the Plasmodium vivax circumsporozoite protein

Details

Serval ID
serval:BIB_ECE8EA6314FE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Use of long synthetic peptides to study the antigenicity and immunogenicity of the Plasmodium vivax circumsporozoite protein
Journal
International Journal for Parasitology
Author(s)
Herrera  S., Bonelo  A., Perlaza  B. L., Valencia  A. Z., Cifuentes  C., Hurtado  S., Quintero  G., Lopez  J. A., Corradin  G., Arevalo-Herrera  M.
ISSN
0020-7519 (Print)
Publication state
Published
Issued date
12/2004
Volume
34
Number
13-14
Pages
1535-46
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Dec
Abstract
Three long synthetic peptides corresponding to amino (N), repeat (R) and carboxyl (C) regions of the Plasmodium vivax circumsporozoite (CS) protein were synthesised and used to assess their potential as vaccine candidates. Antigenicity studies were carried out using human blood samples from residents of a malaria-endemic area of Colombia, and immunogenicity was tested in Aotus monkeys. The N and C peptides spanned the total native amino and carboxyl flanking regions, whereas the R peptide corresponded to a construct based on the first central nona-peptide repeated in tandem three times and colinearly linked to a universal T-cell epitope (ptt-30) derived from tetanus toxin. All three peptides had been shown previously to contain several B-, T-helper (Th) and Cytotoxic T Lymphocytes (CTL) epitopes. Sixty-one percent of the human sera reacted with the R region, whereas 35 and 39% of the samples had antibodies against the N and C peptides, respectively. Human Peripheral Blood Mononuclear Cells (PBMC) showed higher levels of IFN-gamma than IL-4 when stimulated with peptides containing Th epitopes. Aotus monkeys immunised with the peptides formulated in either Montanide ISA720 or Freund's adjuvants produced strong antibody responses that recognised the peptide immunogens and the native circumsporozoite protein on sporozoites. Additionally, high IFN-gamma production was induced when Aotus lymphocytes were stimulated in vitro with each of the three peptides. We observed boosting of antibody responses and IFN-gamma production by exposure to live sporozoites. These results confirm the high antigenicity and immunogenicity of such synthetic polypeptides and underline their vaccine potential.
Keywords
Adolescent Adult Aged Animals Antibodies, Protozoan/biosynthesis Antigens, Protozoan/*immunology Aotidae Child Cytokines/biosynthesis Female Humans Immunization Malaria Vaccines/immunology Male Middle Aged Peptide Fragments/immunology Plasmodium vivax/*immunology Protozoan Proteins/*immunology
Pubmed
Web of science
Create date
24/01/2008 14:55
Last modification date
20/08/2019 16:14
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