Use of isotope ratio mass spectrometry to detect doping with oral testosterone undecanoate: inter-individual variability of 13C/12C ratio.

Details

Serval ID
serval:BIB_ECDD5EBB9AB5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Use of isotope ratio mass spectrometry to detect doping with oral testosterone undecanoate: inter-individual variability of 13C/12C ratio.
Journal
Steroids
Author(s)
Baume N., Saudan C., Desmarchelier A., Strahm E., Sottas P.E., Bagutti C., Cauderay M., Schumacher Y.O., Mangin P., Saugy M.
ISSN
0039-128X
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
71
Number
5
Pages
364-70
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
The metabolic effect of multiple oral testosterone undecanoate (TU) doses over 4 weeks was assessed in seven voluntary men. The protocol was designed to detect accumulation of the substance by choosing the appropriate spot urines collections time and to study the urinary clearance of the substance after weeks of treatment. Urines were analysed by a new GC/C/isotope ratio mass spectrometry (IRMS) method to establish the delta(13)C-values of testosterone metabolites (androsterone and etiocholanolone) together with an endogenous reference compound (16(5alpha)-androsten-3alpha-ol). The significant differences in inter-individual metabolism following TU intake was illustrated by large variations in delta(13)C-values of both T metabolites (maximum Deltadelta(13)C-values = 5.5 per thousand), as well as by very stable longitudinal T/E profiles and carbon isotopic ratios in the first hours following administration. According to T/E ratios and delta(13)C-values, the washout period after 80 mg TU intake was less than 48 h for all subjects and no accumulation phenomenon was observed upon chronic oral administration.
Keywords
Administration, Oral, Adult, Carbon Isotopes, Doping in Sports, Gas Chromatography-Mass Spectrometry, Genetic Variation, Humans, Male, Reference Values, Substance Abuse Detection, Testosterone, Time Factors
Pubmed
Web of science
Create date
16/05/2008 16:25
Last modification date
20/08/2019 16:14
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