Nanomechanical sensor applied to blood culture pellets: a fast approach to determine the antibiotic susceptibility against agents of bloodstream infections.

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Version: Final published version
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Serval ID
serval:BIB_EC311BFF9523
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nanomechanical sensor applied to blood culture pellets: a fast approach to determine the antibiotic susceptibility against agents of bloodstream infections.
Journal
Clinical microbiology and infection
Author(s)
Stupar P. (co-first), Opota O. (co-first), Longo G., Prod'hom G., Dietler G., Greub G., Kasas S.
ISSN
1469-0691 (Electronic)
ISSN-L
1198-743X
Publication state
Published
Issued date
06/2017
Peer-reviewed
Oui
Volume
23
Number
6
Pages
400-405
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The management of bloodstream infection, a life-threatening disease, largely relies on early detection of infecting microorganisms and accurate determination of their antibiotic susceptibility to reduce both mortality and morbidity. Recently we developed a new technique based on atomic force microscopy capable of detecting movements of biologic samples at the nanoscale. Such sensor is able to monitor the response of bacteria to antibiotic's pressure, allowing a fast and versatile susceptibility test. Furthermore, rapid preparation of a bacterial pellet from a positive blood culture can improve downstream characterization of the recovered pathogen as a result of the increased bacterial concentration obtained.
Using artificially inoculated blood cultures, we combined these two innovative procedures and validated them in double-blind experiments to determine the susceptibility and resistance of Escherichia coli strains (ATCC 25933 as susceptible and a characterized clinical isolate as resistant strain) towards a selection of antibiotics commonly used in clinical settings.
On the basis of the variance of the sensor movements, we were able to positively discriminate the resistant from the susceptible E. coli strains in 16 of 17 blindly investigated cases. Furthermore, we defined a variance change threshold of 60% that discriminates susceptible from resistant strains.
By combining the nanomotion sensor with the rapid preparation method of blood culture pellets, we obtained an innovative, rapid and relatively accurate method for antibiotic susceptibility test directly from positive blood culture bottles, without the need for bacterial subculture.
Keywords
Ampicillin/pharmacology, Ampicillin/therapeutic use, Anti-Bacterial Agents/pharmacology, Anti-Bacterial Agents/therapeutic use, Bacteremia/drug therapy, Bacteremia/microbiology, Blood Culture, Ceftriaxone/pharmacology, Ceftriaxone/therapeutic use, Ciprofloxacin/pharmacology, Ciprofloxacin/therapeutic use, Double-Blind Method, Drug Resistance, Bacterial, Escherichia coli/classification, Escherichia coli/drug effects, Escherichia coli Infections/drug therapy, Escherichia coli Infections/microbiology, Humans, Microbial Sensitivity Tests, Microscopy, Atomic Force, Nanotechnology, Atomic force microscopy, Bacteraemia, Bacterial pellet, Bacteriology, Cantilever, Diagnostic microbiology, Nanomechanical sensor, Nanomotion susceptibility test, Rapid analysis
Pubmed
Web of science
Open Access
Yes
Create date
17/01/2017 20:04
Last modification date
10/01/2020 7:18
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