Export of salicylic acid from the chloroplast requires the multidrug and toxin extrusion-like transporter EDS5.

Details

Serval ID
serval:BIB_EBE56335EA4C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Export of salicylic acid from the chloroplast requires the multidrug and toxin extrusion-like transporter EDS5.
Journal
Plant Physiology
Author(s)
Serrano M., Wang B., Aryal B., Garcion C., Abou-Mansour E., Heck S., Geisler M., Mauch F., Nawrath C., Métraux J.P.
ISSN
1532-2548 (Electronic)
ISSN-L
0032-0889
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
162
Number
4
Pages
1815-1821
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Salicylic acid (SA) is central for the defense of plants to pathogens and abiotic stress. SA is synthesized in chloroplasts from chorismic acid by an isochorismate synthase (ICS1); SA biosynthesis is negatively regulated by autoinhibitory feedback at ICS1. Genetic studies indicated that the multidrug and toxin extrusion transporter ENHANCED DISEASE SUSCEPTIBILITY5 (EDS5) of Arabidopsis (Arabidopsis thaliana) is necessary for SA accumulation after biotic and abiotic stress, but so far it is not understood how EDS5 controls the biosynthesis of SA. Here, we show that EDS5 colocalizes with a marker of the chloroplast envelope and that EDS5 functions as a multidrug and toxin extrusion-like transporter in the export of SA from the chloroplast to the cytoplasm in Arabidopsis, where it controls the innate immune response. The location at the chloroplast envelope supports a model of the effect of EDS5 on SA biosynthesis: in the eds5 mutant, stress-induced SA is trapped in the chloroplast and inhibits its own accumulation by autoinhibitory feedback.
Keywords
Arabidopsis/drug effects, Arabidopsis/physiology, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Biological Transport, Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology, Chloroplasts/genetics, Chloroplasts/metabolism, Cytoplasm/genetics, Cytoplasm/metabolism, Immunity, Innate, Membrane Transport Proteins/genetics, Membrane Transport Proteins/metabolism, Molecular Sequence Data, Mutation, Nigericin/pharmacology, Plants, Genetically Modified, Protoplasts/metabolism, Salicylic Acid/metabolism, Ultraviolet Rays
Pubmed
Web of science
Open Access
Yes
Create date
06/10/2016 14:00
Last modification date
20/08/2019 16:14
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