Immune-system-dependent anti-tumor activity of a plant-derived polyphenol rich fraction in a melanoma mouse model.

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Serval ID
serval:BIB_EBCCD2380B13
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immune-system-dependent anti-tumor activity of a plant-derived polyphenol rich fraction in a melanoma mouse model.
Journal
Cell Death and Disease
Author(s)
Gomez-Cadena A., Urueña C., Prieto K., Martinez-Usatorre A., Donda A., Barreto A., Romero P., Fiorentino S.
ISSN
2041-4889 (Electronic)
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
7
Number
6
Pages
e2243
Language
english
Abstract
Recent findings suggest that part of the anti-tumor effects of several chemotherapeutic agents require an intact immune system. This is in part due to the induction of immunogenic cell death. We have identified a gallotannin-rich fraction, obtained from Caesalpinia spinosa (P2Et) as an anti-tumor agent in both breast carcinoma and melanoma. Here, we report that P2Et treatment results in activation of caspase 3 and 9, mobilization of cytochrome c and externalization of annexin V in tumor cells, thus suggesting the induction of apoptosis. This was preceded by the onset of autophagy and the expression of immunogenic cell death markers. We further demonstrate that P2Et-treated tumor cells are highly immunogenic in vaccinated mice and induce immune system activation, clearly shown by the generation of interferon gamma (IFN-γ) producing tyrosine-related protein 2 antigen-specific CD8+ T cells. Moreover, the tumor protective effects of P2Et treatment were abolished in immunodeficient mice, and partially lost after CD4 and CD8 depletion, indicating that P2Et's anti-tumor activity is highly dependent on immune system and at least in part of T cells. Altogether, these results support the hypothesis that the gallotannin-rich fraction P2Et's anti-tumor effects are mediated to a great extent by the endogenous immune response following to the exposure to immunogenic dying tumor cells.
Pubmed
Web of science
Open Access
Yes
Create date
14/06/2016 17:17
Last modification date
20/08/2019 16:13
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