Cutting edge: cytolytic effector function in human circulating CD8+ T cells closely correlates with CD56 surface expression

Details

Serval ID
serval:BIB_EBA789CA2CFC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cutting edge: cytolytic effector function in human circulating CD8+ T cells closely correlates with CD56 surface expression
Journal
Journal of Immunology
Author(s)
Pittet  M. J., Speiser  D. E., Valmori  D., Cerottini  J. C., Romero  P.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
02/2000
Volume
164
Number
3
Pages
1148-52
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb 1
Abstract
Recent data suggest that human effector CD8+ T cells express a distinct CD27-CD45RAhigh (CD57+CD28-CD11ahigh) phenotype. Here, we propose that CTL effector function correlates better with CD56 (neuronal cell adhesion molecule (NCAM)) surface expression. CD56 was absent on cord blood CD8+ T cells, but was expressed by 4-30% of freshly isolated circulating CD8+ T cells from 15 adults. Dramatic oligoclonal expansions in 3/3 individuals were confined to the CD56+ subset of CD8+ T cells. The CD56+ subset generally contained high amounts of intracellular perforin and granzyme B. Finally, direct cytolytic capacity was closely restricted to the CD56+(CD45RAhigh) cells, better than to CD27-CD45RAhigh cells in 5/5 individuals analyzed. Thus, the phenotype corresponding to the circulating effector CD8+ T cell pool may be simplified and more precisely defined by the use of just two surface markers: CD8 and CD56.
Keywords
Adult Antigens, CD56/*biosynthesis Biological Markers/blood CD8-Positive T-Lymphocytes/cytology/*immunology/metabolism Cell Division/immunology Cell Membrane/immunology/metabolism Clone Cells Cytotoxicity, Immunologic/*immunology Granzymes Humans Immunophenotyping Infant, Newborn Membrane Glycoproteins/metabolism Pore Forming Cytotoxic Proteins Serine Endopeptidases/metabolism T-Lymphocyte Subsets/cytology/*immunology/metabolism
Pubmed
Web of science
Create date
28/01/2008 11:13
Last modification date
20/08/2019 16:13
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