Workers exposed to wood dust have an increased micronucleus frequency in nasal and buccal cells: results from a pilot study.

Details

Serval ID
serval:BIB_EB7B11E4B0A4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Workers exposed to wood dust have an increased micronucleus frequency in nasal and buccal cells: results from a pilot study.
Journal
Mutagenesis
Author(s)
Bruschweiler Evin D., Hopf Nancy B., Wild Pascal, Huynh Cong Khanh, Fenech Michael, Thomas Philip, Hor Maryam, Charrière Nicole, Savova-Bianchi Dessislava, Danuser Brigitta
ISSN
1464-3804 (Electronic)
ISSN-L
0267-8357
Publication state
Published
Issued date
05/2014
Peer-reviewed
Oui
Volume
29
Number
3
Pages
201-207
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Wood dust is recognised as a human carcinogen, based on the strong association of wood dust exposure and the elevated risk of malignant tumours of the nasal cavity and paranasal sinuses [sino-nasal cancer (SNC)]. The study aimed to assess genetic damage in workers exposed to wood dust using biomarkers in both buccal and nasal cells that reflect genome instability events, cellular proliferation and cell death frequencies. Nasal and buccal epithelial cells were collected from 31 parquet layers, installers, carpenters and furniture workers (exposed group) and 19 non-exposed workers located in Switzerland. Micronucleus (MN) frequencies were scored in nasal and buccal cells collected among woodworkers. Other nuclear anomalies in buccal cells were measured through the use of the buccal micronucleus cytome assay. MN frequencies in nasal and buccal cells were significantly higher in the exposed group compared to the non-exposed group; odds ratio for nasal cells 3.1 [95% confidence interval (CI) 1.8-5.1] and buccal cells 1.8 (95% CI 1.3-2.4). The exposed group had higher frequencies of cells with nuclear buds, karyorrhectic, pyknotic, karyolytic cells and a decrease in the frequency of basal, binucleated and condensed cells compared to the non-exposed group. Our study confirms that woodworkers have an elevated risk for chromosomal instability in cells of the aerodigestive tract. The MN assay in nasal cells may become a relevant biomonitoring tool in the future for early detection of SNC risk. Future studies should seek to standardise the protocol for MN frequency in nasal cells similar to that for MN in buccal cells.
Keywords
Adult, Case-Control Studies, Chromosomal Instability, Dust, Environmental Monitoring, Humans, Male, Micronucleus Tests/methods, Middle Aged, Mouth Mucosa/pathology, Nasal Mucosa/pathology, Nose Neoplasms/etiology, Occupational Exposure/adverse effects, Paranasal Sinus Neoplasms/etiology, Pilot Projects, Risk Factors, Switzerland, Wood/adverse effects, Young Adult
Pubmed
Web of science
Open Access
Yes
Create date
17/03/2014 17:30
Last modification date
20/08/2019 16:13
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