Perception of root-active CLE peptides requires CORYNE function in the phloem vasculature.

Details

Serval ID
serval:BIB_EB7435DBDC94
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Perception of root-active CLE peptides requires CORYNE function in the phloem vasculature.
Journal
EMBO Reports
Author(s)
Hazak O., Brandt B., Cattaneo P., Santiago J., Rodriguez-Villalon A., Hothorn M., Hardtke C.S.
ISSN
1469-3178 (Electronic)
ISSN-L
1469-221X
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
18
Number
8
Pages
1367-1381
Language
english
Abstract
Arabidopsis root development is orchestrated by signaling pathways that consist of different CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptide ligands and their cognate CLAVATA (CLV) and BARELY ANY MERISTEM (BAM) receptors. How and where different CLE peptides trigger specific morphological or physiological changes in the root is poorly understood. Here, we report that the receptor-like protein CLAVATA 2 (CLV2) and the pseudokinase CORYNE (CRN) are necessary to fully sense root-active CLE peptides. We uncover BAM3 as the CLE45 receptor in the root and biochemically map its peptide binding surface. In contrast to other plant peptide receptors, we found no evidence that SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) proteins act as co-receptor kinases in CLE45 perception. CRN stabilizes BAM3 expression and thus is required for BAM3-mediated CLE45 signaling. Moreover, protophloem-specific CRN expression complements resistance of the crn mutant to root-active CLE peptides, suggesting that protophloem is their principal site of action. Our work defines a genetic framework for dissecting CLE peptide signaling and CLV/BAM receptor activation in the root.

Keywords
CLAVATA, SERK, CLE45, MAKR5, receptor kinase
Pubmed
Web of science
Open Access
Yes
Create date
22/06/2017 16:52
Last modification date
20/08/2019 16:13
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