Immunofluorescence study of actin, acrosin, dynein, tubulin and hyaluronidase and their impact on in-vitro fertilization

Details

Serval ID
serval:BIB_EB729D309A9F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immunofluorescence study of actin, acrosin, dynein, tubulin and hyaluronidase and their impact on in-vitro fertilization
Journal
Human Reproduction
Author(s)
Senn  A., Germond  M., De Grandi  P.
ISSN
0268-1161 (Print)
Publication state
Published
Issued date
07/1992
Volume
7
Number
6
Pages
841-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Abstract
Using polyclonal antibodies, the distribution of actin, acrosin, dynein, tubulin and hyaluronidase has been examined by indirect immunofluorescence in sperm preparations from fertile donors and in-vitro fertilization (IVF) patients. After recording sperm parameters in native semen, spermatozoa were washed free of seminal plasma using either the swim-up or the Percoll filtration technique. Prior to insemination, aliquots of the washed sperm suspensions were prepared for antibody staining. Spermatozoa from fertile donors were analysed in order to establish the specific fluorescence patterns of each antibody and the threshold scores of normality. Immunofluorescence scores obtained from IVF patients were then analysed with respect to IVF outcome. For each tested protein, the number of normal samples were significantly lower in the group which did not fertilize and fertilization rates were significantly reduced when any of the tested proteins were scored as pathological. Normal fluorescence scores were correlated with morphology, motility, velocity and to a lesser extent with sperm concentration in native semen. On the basis of receiver-operating characteristic curves, likelihood ratios and Cohen's kappa values, the presence of acrosin and tubulin yields the most useful information on sperm functional and structural status and on its fertilizing ability.
Keywords
Acrosin/*analysis Actins/*analysis Dynein ATPase/*analysis Female *Fertilization in Vitro Fluorescent Antibody Technique Humans Hyaluronoglucosaminidase/*analysis Male Sperm Count Sperm Motility Spermatozoa/*chemistry/cytology Tubulin/*analysis
Pubmed
Web of science
Create date
25/01/2008 15:40
Last modification date
20/08/2019 16:13
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