Diphenylhydantoin (DPH) blocks HIV-receptor on T-lymphocyte surface.

Details

Serval ID
serval:BIB_EB613C8DF639
Type
Article: article from journal or magazin.
Collection
Publications
Title
Diphenylhydantoin (DPH) blocks HIV-receptor on T-lymphocyte surface.
Journal
Blut
Author(s)
Zimmer J.P., Lehr H.A., Kornhuber M.E., Breitig D., Montagnier L., Gietzen K.
ISSN
0006-5242 (Print)
ISSN-L
0006-5242
Publication state
Published
Issued date
1986
Volume
53
Number
6
Pages
447-450
Language
english
Abstract
Previous reports have shown the capacity of diphenylhydantoin (DPH) to attach to the membranes of lymphatic cells as a hapten and thus exert an unspecific influence on their ability to express certain recognition molecules. This led us to the hypothesis, that DPH might as well serve to manipulate the t-helper-lymphocytes in a way that the mode of infection of these cells by the HIV might be blocked. In order to verify this hypothesis, we exposed normal control lymphocytes as well as lymphocytes from DPH-treated patients (3 X 100-150 mg DPH/day, Phenhydan, for a minimum of 10 days) to radioactively labeled HIV (125I). Remaining radioactivity was assessed using a gamma-counter and measured 64.000-92.000 counts/min (n = 24, mean 80.000) for the control lymphocytes, while remaining radioactivity for the DPH-treated lymphocytes ranged between 2000 and 7000 counts/min (n = 24, mean 4.000, p less than 0.001). These results and similar experiments obtained with FITC-labeled HIV led us to the conclusion that DPH inhibits HIV recognition of T-lymphocytes and therefore might be used in therapy and prophylaxis of AIDS.
Keywords
Cells, Cultured, HIV/drug effects, HIV/metabolism, Humans, Iodine Radioisotopes/diagnostic use, Phenytoin/pharmacology, Receptors, Virus/drug effects, Receptors, Virus/metabolism, T-Lymphocytes/drug effects
Pubmed
Create date
27/11/2011 19:07
Last modification date
20/08/2019 16:13
Usage data