Different mobilization of calcium in endothelin-1-induced contractions in human arteries and veins: effects of calcium antagonists

Details

Serval ID
serval:BIB_EB426DD02507
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Different mobilization of calcium in endothelin-1-induced contractions in human arteries and veins: effects of calcium antagonists
Journal
Journal of Cardiovascular Pharmacology
Author(s)
Yang  Z., Bauer  E., von Segesser  L., Stulz  P., Turina  M., Luscher  T. F.
ISSN
0160-2446
Publication state
Published
Issued date
10/1990
Peer-reviewed
Oui
Volume
16
Number
4
Pages
654-60
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
We studied the role of extra- and intracellular Ca2+ in endothelin-1-induced contractions of the isolated human internal mammary artery and vein. Veins were more sensitive to the peptide than arteries (concentration shift:3.2-fold; n = 4-10, p less than 0.05). The Ca2+ antagonists darodipine, verapamil, and diltiazem (10(-7)-10(-6) M) did not prevent the response to endothelin-1 in both vessels. In contrast, darodipine (10(-8)-10(-6) M), added after the contraction had developed, partially reversed the response in the artery (26 +/- 7%) and particularly in the vein (67 +/- 5%; n = 4, p less than 0.005 compared to the artery). Removal of extracellular Ca2+ reduced the contractions to endothelin-1 (10(-8) M) in the artery (control: 89 +/- 4% of 100 mM KCl; Ca2(+)-free: 68 +/- 4% n = 4-6, p less than 0.01), but not in the vein except at low concentrations (10(-9) M) of the peptide. After removal of intracellular Ca2+ with caffeine in the artery, endothelin-1 still evoked a contraction (17 +/- 3%, n = 3; p less than 0.005 vs. control), while in the vein the response was abolished. Thus, mobilization of Ca2+ during endothelin-1-induced contractions differs in the human internal mammary artery and vein. In the artery, the contraction depends on extracellular Ca2+, intracellular caffeine-sensitive Ca2+ stores, and a caffeine-insensitive component, while in veins, mobilization of intracellular Ca2+ is most important. Ca2+ antagonists do not prevent, but partially reverse, endothelin-1-induced contractions indicating that voltage-operated Ca2+ channels do not initiate but contribute to the maintenance of the response.
Keywords
Caffeine/pharmacology Calcium Channel Blockers/*pharmacology Calcium Channels/*drug effects Endothelins/*pharmacology Humans Mammary Arteries Muscle Contraction/*drug effects Veins
Pubmed
Web of science
Create date
14/02/2008 14:19
Last modification date
20/08/2019 16:13
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