Timing of gene expression in a cell-fate decision system.

Details

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State: Public
Version: Final published version
Serval ID
serval:BIB_EB2E2F5249EF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Timing of gene expression in a cell-fate decision system.
Journal
Molecular systems biology
Author(s)
Aymoz D., Solé C., Pierre J.J., Schmitt M., de Nadal E., Posas F., Pelet S.
ISSN
1744-4292 (Electronic)
ISSN-L
1744-4292
Publication state
Published
Issued date
25/04/2018
Peer-reviewed
Oui
Volume
14
Number
4
Pages
e8024
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
During development, morphogens provide extracellular cues allowing cells to select a specific fate by inducing complex transcriptional programs. The mating pathway in budding yeast offers simplified settings to understand this process. Pheromone secreted by the mating partner triggers the activity of a MAPK pathway, which results in the expression of hundreds of genes. Using a dynamic expression reporter, we quantified the kinetics of gene expression in single cells upon exogenous pheromone stimulation and in the physiological context of mating. In both conditions, we observed striking differences in the timing of induction of mating-responsive promoters. Biochemical analyses and generation of synthetic promoter variants demonstrated how the interplay between transcription factor binding and nucleosomes contributes to determine the kinetics of transcription in a simplified cell-fate decision system.
Keywords
Cell Differentiation/genetics, Cell Lineage/genetics, Gene Expression Regulation, Fungal/genetics, Genes, Mating Type, Fungal/genetics, MAP Kinase Signaling System/genetics, Pheromones/genetics, Promoter Regions, Genetic, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics, Single-Cell Analysis, MAPK pathway, gene expression, single‐cell measurements, yeast mating
Pubmed
Web of science
Open Access
Yes
Create date
03/05/2018 19:22
Last modification date
20/08/2019 17:13
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