Dynamic molecular combing: stretching the whole human genome for high-resolution studies

Details

Serval ID
serval:BIB_EB0CF9F934D6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dynamic molecular combing: stretching the whole human genome for high-resolution studies
Journal
Science
Author(s)
Michalet  X., Ekong  R., Fougerousse  F., Rousseaux  S., Schurra  C., Hornigold  N., van Slegtenhorst  M., Wolfe  J., Povey  S., Beckmann  J. S., Bensimon  A.
ISSN
0036-8075 (Print)
Publication state
Published
Issued date
09/1997
Volume
277
Number
5331
Pages
1518-23
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 5
Abstract
DNA in amounts representative of hundreds of eukaryotic genomes was extended on silanized surfaces by dynamic molecular combing. The precise measurement of hybridized DNA probes was achieved directly without requiring normalization. This approach was validated with the high-resolution mapping of cosmid contigs on a yeast artificial chromosome (YAC) within yeast genomic DNA. It was extended to human genomic DNA for precise measurements ranging from 7 to 150 kilobases, of gaps within a contig, and of microdeletions in the tuberous sclerosis 2 gene on patients' DNA. The simplicity, reproducibility, and precision of this approach makes it a powerful tool for a variety of genomic studies.
Keywords
Calpain/genetics Chromosome Mapping/*methods Chromosomes, Artificial, Yeast Cloning, Molecular Cosmids DNA Probes Electrophoresis, Gel, Pulsed-Field *Genetic Techniques *Genome, Fungal *Genome, Human Humans In Situ Hybridization, Fluorescence Isoenzymes/genetics *Muscle Proteins Muscular Dystrophies/genetics Mutation Proteins/genetics Repressor Proteins/genetics Reproducibility of Results Sequence Deletion Silanes Tuberous Sclerosis/genetics Tumor Suppressor Proteins
Pubmed
Web of science
Create date
25/01/2008 16:17
Last modification date
20/08/2019 16:13
Usage data