Dynamic molecular combing: stretching the whole human genome for high-resolution studies
Details
Serval ID
serval:BIB_EB0CF9F934D6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dynamic molecular combing: stretching the whole human genome for high-resolution studies
Journal
Science
ISSN
0036-8075 (Print)
Publication state
Published
Issued date
09/1997
Volume
277
Number
5331
Pages
1518-23
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 5
Research Support, Non-U.S. Gov't --- Old month value: Sep 5
Abstract
DNA in amounts representative of hundreds of eukaryotic genomes was extended on silanized surfaces by dynamic molecular combing. The precise measurement of hybridized DNA probes was achieved directly without requiring normalization. This approach was validated with the high-resolution mapping of cosmid contigs on a yeast artificial chromosome (YAC) within yeast genomic DNA. It was extended to human genomic DNA for precise measurements ranging from 7 to 150 kilobases, of gaps within a contig, and of microdeletions in the tuberous sclerosis 2 gene on patients' DNA. The simplicity, reproducibility, and precision of this approach makes it a powerful tool for a variety of genomic studies.
Keywords
Calpain/genetics
Chromosome Mapping/*methods
Chromosomes, Artificial, Yeast
Cloning, Molecular
Cosmids
DNA Probes
Electrophoresis, Gel, Pulsed-Field
*Genetic Techniques
*Genome, Fungal
*Genome, Human
Humans
In Situ Hybridization, Fluorescence
Isoenzymes/genetics
*Muscle Proteins
Muscular Dystrophies/genetics
Mutation
Proteins/genetics
Repressor Proteins/genetics
Reproducibility of Results
Sequence Deletion
Silanes
Tuberous Sclerosis/genetics
Tumor Suppressor Proteins
Pubmed
Web of science
Create date
25/01/2008 16:17
Last modification date
20/08/2019 16:13