Spatial coupling between DNA replication and mismatch repair in Caulobacter crescentus.

Details

Serval ID
serval:BIB_EAACA80E11E2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Spatial coupling between DNA replication and mismatch repair in Caulobacter crescentus.
Journal
Nucleic acids research
Author(s)
Chai T., Terrettaz C., Collier J.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Publication state
Published
Issued date
06/04/2021
Peer-reviewed
Oui
Volume
49
Number
6
Pages
3308-3321
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The DNA mismatch repair (MMR) process detects and corrects replication errors in organisms ranging from bacteria to humans. In most bacteria, it is initiated by MutS detecting mismatches and MutL nicking the mismatch-containing DNA strand. Here, we show that MMR reduces the appearance of rifampicin resistances more than a 100-fold in the Caulobacter crescentus Alphaproteobacterium. Using fluorescently-tagged and functional MutS and MutL proteins, live cell microscopy experiments showed that MutS is usually associated with the replisome during the whole S-phase of the C. crescentus cell cycle, while MutL molecules may display a more dynamic association with the replisome. Thus, MMR components appear to use a 1D-scanning mode to search for rare mismatches, although the spatial association between MutS and the replisome is dispensible under standard growth conditions. Conversely, the spatial association of MutL with the replisome appears as critical for MMR in C. crescentus, suggesting a model where the β-sliding clamp licences the endonuclease activity of MutL right behind the replication fork where mismatches are generated. The spatial association between MMR and replisome components may also play a role in speeding up MMR and/or in recognizing which strand needs to be repaired in a variety of Alphaproteobacteria.
Keywords
Amino Acid Motifs, Base Pair Mismatch, Caulobacter crescentus/genetics, Caulobacter crescentus/metabolism, DNA Helicases/metabolism, DNA Mismatch Repair, DNA Replication, DNA-Directed DNA Polymerase/metabolism, Multienzyme Complexes/metabolism, MutL Proteins/metabolism, MutS DNA Mismatch-Binding Protein/chemistry, MutS DNA Mismatch-Binding Protein/metabolism, S Phase/genetics
Pubmed
Web of science
Open Access
Yes
Funding(s)
Swiss National Science Foundation / 31003A_173075
Create date
09/03/2021 17:55
Last modification date
29/05/2021 6:31
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