High yield of cells committed to the photoreceptor fate from expanded mouse retinal stem cells

Details

Serval ID
serval:BIB_EA56EB4EF5B1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
High yield of cells committed to the photoreceptor fate from expanded mouse retinal stem cells
Journal
Stem Cells
Author(s)
Merhi-Soussi  F., Angenieux  B., Canola  K., Kostic  C., Tekaya  M., Hornfeld  D., Arsenijevic  Y.
ISSN
1066-5099 (Print)
Publication state
Published
Issued date
09/2006
Volume
24
Number
9
Pages
2060-70
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Abstract
The purpose of the present work was to generate, from retinal stem cells (RSCs), a large number of cells committed toward the photoreceptor fate in order to provide an unlimited cell source for neurogenesis and transplantation studies. We expanded RSCs (at least 34 passages) sharing characteristics of radial glial cells and primed the cells in vitro with fibroblast growth factor (FGF)-2 for 5 days, after which cells were treated with the B27 supplement to induce cell differentiation and maturation. Upon differentiation, cells expressed cell type-specific markers corresponding to neurons and glia. We show by immunocytochemistry analysis that a subpopulation of differentiated cells was committed to the photoreceptor lineage given that these cells expressed the photoreceptor proteins recoverin, peripherin, and rhodopsin in a same ratio. Furthermore, cells infected during the differentiation procedure with a lentiviral vector expressing green fluorescent protein (GFP) under the control of either the rhodopsin promoter or the interphotoreceptor retinoid-binding protein (IRBP) promoter, expressed GFP. FGF-2 priming increased neuronal differentiation while decreasing glia generation. Reverse transcription-polymerase chain reaction analyses revealed that the differentiated cells expressed photoreceptor-specific genes such as Crx, rhodopsin, peripherin, IRBP, and phosphodiesterase-alpha. Quantification of the differentiated cells showed a robust differentiation into the photoreceptor lineage: Approximately 25%-35% of the total cells harbored photoreceptor markers. The generation of a significant number of nondifferentiated RSCs as well as differentiated photoreceptors will enable researchers to determine via transplantation studies which cells are the most adequate to integrate a degenerating retina.
Keywords
Animals Biological Markers/metabolism Cell Count *Cell Differentiation *Cell Lineage Cell Proliferation Gene Expression Regulation Intermediate Filament Proteins/metabolism Mice Nerve Tissue Proteins/metabolism Neuroglia/cytology Neurons/cytology Photoreceptors/*cytology RNA, Messenger/genetics/metabolism Retina/*cytology Stem Cells/*cytology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:31
Last modification date
20/08/2019 16:12
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