Validation of an LC-MS/MS method for urinary homovanillic and vanillylmandelic ACIDS and application to the diagnosis of neuroblastoma.

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Version: Final published version
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_EA11597B08A8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Validation of an LC-MS/MS method for urinary homovanillic and vanillylmandelic ACIDS and application to the diagnosis of neuroblastoma.
Journal
Journal of mass spectrometry and advances in the clinical lab
Author(s)
Rossi L., Matser YAH, Barco S., Cafaro A., Pigliasco F., Biondi M., Mancin F., van der Ham M., de Sain-van der Velden MGM, Ash S., Popovic M.B., van Kuilenburg ABP, Conte M., Garaventa A., Tytgat GAM, Cangemi G.
ISSN
2667-145X (Electronic)
ISSN-L
2667-145X
Publication state
Published
Issued date
04/2025
Peer-reviewed
Oui
Volume
36
Pages
73-81
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Urinary catecholamine metabolites are well-established biomarkers for neuroblastoma (NB). Homovanillic acid (HVA) and vanillylmandelic acid (VMA) are the most frequently measured metabolites within SIOPEN - Catecholamine Working Group laboratories. Here, we evaluated the performance of a new LC-MS/MS in vitro diagnostic (IVD) kit for HVA and VMA to facilitate inter-laboratory harmonization.
HVA and VMA and their deuterated internal standards were analyzed with a commercial method, on a ThermoFisher Quantiva LC-MS/MS. Validation was performed first using internal quality control and external quality assessment (IQC and EQA) samples. Next by clinical validation on 120 samples, previously tested by HPLC-ECD. Finally, 36 samples were exchanged between SIOPEN reference laboratories and analyzed by three methods.
Using QCs and EQA the method was validated in a wide calibration range (4.61-830 µmol/L for HVA and 4.44-800 µmol/L for VMA). Intra-day CVs (n = 5) were 7 and 8 % for HVA and 5 and 6 % for VMA for QC low and QC high, respectively; Inter-day CV% were 7 and 3 % for HVA and 2 and 7 % for VMA at QC low and QC high, respectively. Its application to 120 clinical samples confirmed a high diagnostic accuracy. The inter-laboratory quality control assessment showed interchangeable results (p = 0,73 and p = 0.15 for HVA and VMA, respectively).
The LC-MS/MS IVD method could be considered a useful tool for clinical laboratories involved in the measurement of catecholamines, contributing to harmonization efforts.
Keywords
Homovanillic acid, Inter-laboratory harmonization, Liquid Chromatography-tandem Mass Spectrometry, Neuroblastoma, Pediatrics, Urinary catecholamine, Vanillylmandelic acid
Pubmed
Web of science
Open Access
Yes
Create date
23/05/2025 9:37
Last modification date
03/07/2025 7:06
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