Developmental changes in the heavy subunit of neurofilaments in the corpus callosum of the cat.

Details

Serval ID
serval:BIB_E95C493E1616
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Developmental changes in the heavy subunit of neurofilaments in the corpus callosum of the cat.
Journal
Brain Research. Developmental Brain Research
Author(s)
Guadano-Ferraz A., Riederer B.M., Innocenti G.M.
ISSN
0165-3806 (Print)
ISSN-L
0165-3806
Publication state
Published
Issued date
1990
Volume
56
Number
2
Pages
244-256
Language
english
Abstract
In the corpus callosum of the cat, the heavy subunit of neurofilaments (NFH) can be demonstrated with the monoclonal antibody NE14, as early as P11, not at P3, and only in a few axons. At P18-19 and more markedly at P29, many more callosal axons have become positive to NE14 and this is similar to what is found in the adult. In contrast, callosal axons become positive to the neurofilament antibody SMI-32 only between P29 and P39 and remain positive in the adult. Treatment with alkaline phosphatase prevents axonal staining with NE14, but results in SMI-32 staining of a few callosal axons as early as P11, but not at P3. Between P11 and P19 the number of axons stained with SMI-32 after alkaline phosphatase treatment increases, in parallel with that of axons stained with NE14. Thus NE14 appears to recognize a phosphorylated form of NFH, while SMI-32 appears to recognize an epitope of NFH which is either masked by phosphate or inaccessible until between P29 and P39, unless the tissue is treated with alkaline phosphatase. These two forms of NFH appear towards the end of the period of massive developmental elimination of callosal axons. They are also synchronous with changes in the spacing of neurofilaments quantified in a separate ultrastructural study. These cytoskeletal changes may terminate the juvenile-labile state of callosal axons and allow further axial growth of the axon.
Keywords
Aging, Alkaline Phosphatase, Animals, Animals, Newborn, Axons/ultrastructure, Cats, Corpus Callosum/cytology, Corpus Callosum/embryology, Embryo, Mammalian, Intermediate Filament Proteins/analysis, Macromolecular Substances, Neurofilament Proteins
Pubmed
Web of science
Create date
24/01/2008 14:34
Last modification date
20/08/2019 16:11
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