Amoeba-resisting bacteria and ventilator-associated pneumonia

Details

Serval ID
serval:BIB_E936FBF0C39D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Amoeba-resisting bacteria and ventilator-associated pneumonia
Journal
Emerging Infectious Diseases
Author(s)
La Scola  B., Boyadjiev  I., Greub  G., Khamis  A., Martin  C., Raoult  D.
ISSN
1080-6040 (Print)
Publication state
Published
Issued date
07/2003
Volume
9
Number
7
Pages
815-21
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Abstract
To evaluate the role of amoeba-associated bacteria as agents of ventilator-associated pneumonia (VAP), we tested the water from an intensive care unit (ICU) every week for 6 months for such bacteria isolates; serum samples and bronchoalveolar lavage samples (BAL) were also obtained from 30 ICU patients. BAL samples were examined for amoeba-associated bacteria DNA by suicide-polymerase chain reaction, and serum samples were tested against ICU amoeba-associated bacteria. A total of 310 amoeba-associated bacteria from 10 species were isolated. Twelve of 30 serum samples seroconverted to one amoeba-associated bacterium isolated in the ICU, mainly Legionella anisa and Bosea massiliensis, the most common isolates from water (p=0.021). Amoeba-associated bacteria DNA was detected in BAL samples from two patients whose samples later seroconverted. Seroconversion was significantly associated with VAP and systemic inflammatory response syndrome, especially in patients for whom no etiologic agent was found by usual microbiologic investigations. Amoeba-associated bacteria might be a cause of VAP in ICUs, especially when microbiologic investigations are negative.
Keywords
Adult Amoeba/*isolation & purification/*microbiology Animals Bacteria/*classification/*isolation & purification Bronchoalveolar Lavage Fluid/microbiology/parasitology Female Humans Intensive Care Units Male Pneumonia/*microbiology/parasitology Ventilators, Mechanical/adverse effects/*microbiology/parasitology Water Microbiology
Pubmed
Web of science
Create date
25/01/2008 14:27
Last modification date
20/08/2019 16:11
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