Intraoperative photodynamic therapy of the chest cavity in malignant pleural mesothelioma bearing rats.

Details

Serval ID
serval:BIB_E8EAF79640BD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Intraoperative photodynamic therapy of the chest cavity in malignant pleural mesothelioma bearing rats.
Journal
Lasers in Surgery and Medicine
Author(s)
Krueger T., Pan Y., Tran N., Altermatt H.J., Opitz I., Ris H.B.
ISSN
0196-8092 (Print)
ISSN-L
0196-8092
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
37
Number
4
Pages
271-277
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
BACKGROUND AND OBJECTIVE: Experimental assessment of anticancer effect, normal tissue damage, and toxicity of intrathoracic mTHPC-mediated photodynamic therapy (PDT) combined to surgery in malignant pleural mesothelioma (MPM) bearing rats.
STUDY DESIGN/MATERIALS AND METHODS: Six days after implantation of syngenic malignant mesothelioma cells in the left chest cavity of Fischer rats (n = 21) and 4 days after sensitization (0.1 mg/kg mTHPC), a left-sided pneumonectomy was performed, followed by intraoperative light delivery (652 nm, fluence 20 J/cm(2)), either by spherical illumination of the chest cavity (fluence rate 15 mW/cm(2)) or by focal illumination of a tumor area (fluence rate 150 mW/cm(2)). Controls comprised tumor-bearing untreated animals, tumor-bearing animals undergoing pneumonectomy, and tumor-bearing animals undergoing pneumonectomy and light delivery without sensitization or sensitization without light delivery. No thoracocentesis was performed during follow-up.
RESULTS: An invasively growing sarcomatous type of mesothelioma was found in all animals at day 10, without tumor necrosis in control animals. PDT resulted in 0.5-1 mm deep inhomogeneous tumor necrosis after spherical, and in a 1-2 mm deep tumor necrosis after focal illumination. No injury to mediastinal organs was observed, neither after PDT with spherical nor with focal light delivery except focal interstitial lung fibrosis at the mediastinal area of the opposite lung. All animals with pneumonectomy followed by spherical PDT of the entire tumor-bearing chest cavity died within 72 hours whereas all other animals survived. All animals that died presented massive pleural effusion.
CONCLUSIONS: PDT following pneumonectomy in mesothelioma bearing rats was technically feasible and allowed to study its effect on tumor and normal tissues. PDT-related tumor necrosis was observed after spherical and focal light delivery, however, pneumonectomy followed by PDT with spherical light delivery to the tumor-bearing chest cavity resulted in fatal complications.
Keywords
Animals, Antineoplastic Agents/therapeutic use, Cell Line, Tumor, Combined Modality Therapy, Intraoperative Period, Male, Mesoporphyrins/therapeutic use, Mesothelioma/drug therapy, Mesothelioma/pathology, Neoplasm Transplantation, Photochemotherapy, Photosensitizing Agents/therapeutic use, Pleural Neoplasms/drug therapy, Pleural Neoplasms/pathology, Pneumonectomy, Rats, Rats, Inbred F344
Pubmed
Web of science
Create date
29/01/2008 12:59
Last modification date
20/08/2019 16:11
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