A comprehensive assessment of demographic, environmental, and host genetic associations with gut microbiome diversity in healthy individuals.

Details

Serval ID
serval:BIB_E8404D993AE8
Type
Article: article from journal or magazin.
Collection
Publications
Title
A comprehensive assessment of demographic, environmental, and host genetic associations with gut microbiome diversity in healthy individuals.
Journal
Microbiome
Author(s)
Scepanovic P., Hodel F., Mondot S., Partula V., Byrd A., Hammer C., Alanio C., Bergstedt J., Patin E., Touvier M., Lantz O., Albert M.L., Duffy D., Quintana-Murci L., Fellay J.
Working group(s)
Milieu Intérieur Consortium
Contributor(s)
Abel L., Alcover A., Aschard H., Astrom K., Bousso P., Bruhns P., Cumano A., Demangel C., Deriano L., Di Santo J., Dromer F., Duffy D., Eberl G., Enninga J., Fellay J., Gelpi O., Gomperts-Boneca I., Hasan M., Hercberg S., Lantz O., Leclerc C., Mouquet H., Pellegrini S., Pol S., Rausell A., Rogge L., Sakuntabhai A., Schwartz O., Schwikowski B., Shorte S., Soumelis V., Tangy F., Tartour E., Toubert A., Touvier M., Ungeheuer M.N., Albert M.L., Quintana-Murci L.
ISSN
2049-2618 (Electronic)
ISSN-L
2049-2618
Publication state
Published
Issued date
13/09/2019
Peer-reviewed
Oui
Volume
7
Number
1
Pages
130
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
The gut microbiome is an important determinant of human health. Its composition has been shown to be influenced by multiple environmental factors and likely by host genetic variation. In the framework of the Milieu Intérieur Consortium, a total of 1000 healthy individuals of western European ancestry, with a 1:1 sex ratio and evenly stratified across five decades of life (age 20-69), were recruited. We generated 16S ribosomal RNA profiles from stool samples for 858 participants. We investigated genetic and non-genetic factors that contribute to individual differences in fecal microbiome composition.
Among 110 demographic, clinical, and environmental factors, 11 were identified as significantly correlated with α-diversity, ß-diversity, or abundance of specific microbial communities in multivariable models. Age and blood alanine aminotransferase levels showed the strongest associations with microbiome diversity. In total, all non-genetic factors explained 16.4% of the variance. We then searched for associations between > 5 million single nucleotide polymorphisms and the same indicators of fecal microbiome diversity, including the significant non-genetic factors as covariates. No genome-wide significant associations were identified after correction for multiple testing. A small fraction of previously reported associations between human genetic variants and specific taxa could be replicated in our cohort, while no replication was observed for any of the diversity metrics.
In a well-characterized cohort of healthy individuals, we identified several non-genetic variables associated with fecal microbiome diversity. In contrast, host genetics only had a negligible influence. Demographic and environmental factors are thus the main contributors to fecal microbiome composition in healthy individuals.
ClinicalTrials.gov identifier NCT01699893.
Keywords
Adult, Aged, Bacteria/classification, Bacteria/isolation & purification, Cohort Studies, Demography, Environment, Feces/microbiology, Female, Gastrointestinal Microbiome/genetics, Healthy Volunteers, Humans, Life Style, Male, Middle Aged, Young Adult, 16S rRNA gene sequencing, Demographics, GWAS, Genomics, Gut, Healthy, Human, Microbiome
Pubmed
Web of science
Open Access
Yes
Create date
20/09/2019 23:14
Last modification date
15/07/2020 6:26
Usage data