Viral Double-Stranded RNA Detection by DNase I and Nuclease S1 digestions in Leishmania parasites.

Details

Ressource 1Request a copy Sous embargo indéterminé.
State: Public
Version: author
License: Not specified
Serval ID
serval:BIB_E819D1295762
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Viral Double-Stranded RNA Detection by DNase I and Nuclease S1 digestions in Leishmania parasites.
Journal
Bio-protocol
Author(s)
Isorce N., Fasel N.
ISSN
2331-8325 (Electronic)
ISSN-L
2331-8325
Publication state
Published
Issued date
05/05/2020
Peer-reviewed
Oui
Volume
10
Number
9
Pages
e3598
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Many RNA viruses are found in protozoan parasites. They can be responsible for more serious pathology or treatment failure. For the detection of viral double-stranded RNA (dsRNA), sequence-dependent and -independent methods are available, such as quantitative real-time PCR and immunofluorescence, dot blot, ELISA or sequencing. The technique presented here is sequence-independent and is well detailed in the following protocol, taking the example of Leishmania RNA virus (LRV) in Leishmania guyanensis (Lgy) species. To summarise, the protocol is divided into four major steps: RNA extraction from the parasites, RNA purification, enzymatic digestions with DNase I and Nuclease S1, and visualization by gel electrophoresis. This method can be used to detect other viral dsRNA in other parasites. It provides an additional tool, complementary to other techniques previously cited and it is easy and quite fast to achieve.
Keywords
DNase I, Detection, LRV (Leishmania RNA virus), Nuclease S1, Parasite, Virus, dsRNA
Pubmed
Web of science
Open Access
Yes
Create date
31/08/2020 12:52
Last modification date
17/03/2021 6:26
Usage data