In vitro negative selection of viral superantigen-reactive thymocytes by thymic dendritic cells.

Details

Serval ID
serval:BIB_E7F406C07E1D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vitro negative selection of viral superantigen-reactive thymocytes by thymic dendritic cells.
Journal
Blood
Author(s)
Ferrero I., Anjuère F., MacDonald H.R., Ardavín C.
ISSN
0006-4971
Publication state
Published
Issued date
1997
Peer-reviewed
Oui
Volume
90
Number
5
Pages
1943-1951
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Intrathymic expression of endogenous mouse mammary tumor virus (MMTV)-encoded superantigens (SAg) induces the clonal deletion of T cells bearing SAg-reactive T-cell receptor (TCR) Vbeta elements. However, the identity of the thymic antigen-presenting cells (APC) involved in the induction of SAg tolerance remains to be defined. We have analyzed the potential of dendritic cells (DC) to mediate the clonal deletion of Mtv-7-reactive TCR alphabeta P14 transgenic thymocytes in an in vitro assay. Our results show that both thymic and splenic DC induced the deletion of TCR transgenic double positive (DP) thymocytes. DC appear to be more efficient than splenic B cells as negatively selecting APC in this experimental system. Interestingly, thymic and splenic DC display a differential ability to induce CD4+ SP thymocyte proliferation. These observations suggest that thymic DC may have an important role in the induction of SAg tolerance in vivo.
Keywords
Animals, Antigen Presentation, Cell Differentiation/immunology, Cells, Cultured, Clone Cells, Dendritic Cells/immunology, Gene Deletion, Lymphocyte Activation/immunology, Mammary Tumor Virus, Mouse/immunology, Mice, Mice, Inbred BALB C, Mice, Transgenic, Receptors, Antigen, T-Cell, alpha-beta/genetics, Receptors, Antigen, T-Cell, alpha-beta/immunology, Superantigens/immunology, T-Lymphocytes/immunology, Thymus Gland/immunology
Pubmed
Web of science
Create date
11/02/2010 11:02
Last modification date
20/08/2019 16:10
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