Chip electrospray mass spectrometry for carbohydrate analysis.

Details

Serval ID
serval:BIB_E78F61A0238B
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Chip electrospray mass spectrometry for carbohydrate analysis.
Journal
Electrophoresis
Author(s)
Zamfir A.D., Bindila L., Lion N., Allen M., Girault H.H., Peter-Katalinić J.
ISSN
0173-0835 (Print)
ISSN-L
0173-0835
Publication state
Published
Issued date
2005
Volume
26
Number
19
Pages
3650-3673
Language
english
Notes
Publication types: Journal Article ; Review Publication Status: ppublish
Abstract
Currently two types of chip systems are used in conjunction with MS: out-of-plane devices, where hundreds of nozzles, nanospray emitters are integrated onto a single silicon substrate from which electrospray is established perpendicular to the substrate, and planar microchips, embedding a microchannel at the end of which electrospray is generated in-plane, on the edge of the microchip. In the last two years, carbohydrate research greatly benefited from the introduction and implementation of the chip-based MS. In two laboratories the advantages of the chip electrospray in terms of ionization efficiency, sensitivity, reproducibility, quality of data in combination with high mass accuracy, and resolution of detection were systematically explored for several carbohydrate classes: O- and N-glycopeptides, oligosaccharides, gangliosides and glycoprotein-derived O- and N-glycans, and glycopeptides. The current state-of-the-art in interfacing the chip electrospray devices to high-performance MS for carbohydrate analysis, and the particular requirements for method optimization in both positive and negative ion modes are reviewed here. The recent applications of these miniaturized devices and their general potential for glycomic-based surveys are highlighted.
Keywords
Amino Acid Sequence, Carbohydrate Sequence, Carbohydrates/analysis, Microfluidic Analytical Techniques/instrumentation, Molecular Sequence Data, Polymers/chemistry, Spectrometry, Mass, Electrospray Ionization/instrumentation, Spectrometry, Mass, Electrospray Ionization/methods
Pubmed
Web of science
Create date
09/11/2014 22:47
Last modification date
20/08/2019 16:10
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