Differential effects of the protein kinase inhibitor K-252a on the in vitro survival of chick embryonic neurons.

Details

Serval ID
serval:BIB_E7734B1F4539
Type
Article: article from journal or magazin.
Collection
Publications
Title
Differential effects of the protein kinase inhibitor K-252a on the in vitro survival of chick embryonic neurons.
Journal
Neuroscience Letters
Author(s)
Borasio G.D.
ISSN
0304-3940 (Print)
ISSN-L
0304-3940
Publication state
Published
Issued date
1990
Volume
108
Number
1-2
Pages
207-212
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
The effects of the protein kinase inhibitor K-252a on the in vitro survival of different populations of chick embryonic neurons were tested. Following dissociation from the corresponding ganglia by trituration, the neurons were cultured on laminin-coated dishes in both the presence and absence of their respective neurotrophic factor. K-252a alone promoted long-term neuronal survival of dorsal root ganglion (DRG) and ciliary ganglion (CG) neurons in a dose-dependent fashion. No such effect was seen with sympathetic ganglion (SG) neurons. In addition, K-252a did not interfere with nerve growth factor (NGF)- or ciliary neuronotrophic factor (CNTF)-mediated survival of DRG or CG neurons, respectively, but completely blocked the NGF- and CNTF-induced survival of SG neurons. High potassium-induced survival of SG neurons was not affected by K-252a. These results point to differences between various neuronal populations in the signal transduction mechanism for neurotrophic factors.
Keywords
Animals, Carbazoles/pharmacology, Cell Survival/drug effects, Cells, Cultured, Chick Embryo, Enzyme Inhibitors/pharmacology, Ganglia, Parasympathetic/cytology, Ganglia, Parasympathetic/drug effects, Ganglia, Sympathetic/cytology, Ganglia, Sympathetic/drug effects, Indole Alkaloids, Protein Kinases/metabolism
Pubmed
Web of science
Create date
13/01/2014 16:43
Last modification date
20/08/2019 16:10
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