Visualizing intermolecular interactions in T cells.
Details
State: Public
Version: author
Serval ID
serval:BIB_E76961E72E99
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Visualizing intermolecular interactions in T cells.
Journal
Current Topics in Microbiology and Immunology
ISSN
0070-217X (Print)
ISSN-L
0070-217X
Publication state
Published
Issued date
2009
Volume
334
Pages
31-46
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review Publication Status: ppublish
Abstract
The use of appropriate fluorescent proteins has allowed the use of FRET microscopy for investigation of intermolecular interactions in living cells. This method has the advantage of both being dynamic and of working at the subcellular level, so that the time and place where proteins interact can be visualized. We have used FRET microscopy to analyze the interactions between the T cell antigen receptor and the coreceptors CD4 and CD8. This chapter reviews data on how these coreceptors are recruited to the immunological synapse, and how they interact when the T cell is stimulated by different ligands.
Keywords
Animals, Antigen Presentation, Antigens, CD4/metabolism, Antigens, CD8/metabolism, Fluorescence Resonance Energy Transfer/methods, Humans, Nanotubes, Receptors, Antigen, T-Cell/metabolism, T-Lymphocytes/immunology, T-Lymphocytes/metabolism
Pubmed
Web of science
Create date
28/05/2013 11:32
Last modification date
20/08/2019 17:10
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