Determination of nalbuphine using high-performance liquid chromatography coupled to photodiode-array detection and gas chromatography coupled to mass spectrometry.

Details

Serval ID
serval:BIB_E741E8FD179E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Determination of nalbuphine using high-performance liquid chromatography coupled to photodiode-array detection and gas chromatography coupled to mass spectrometry.
Journal
Journal of Chromatography
Author(s)
Kintz P., Tracqui A., Mangin P.
ISSN
0021-9673[print], 0021-9673[linking]
Publication state
Published
Issued date
1992
Volume
579
Number
1
Pages
172-176
Language
english
Abstract
A procedure involving capillary column gas chromatography coupled to mass spectrometry and a method involving liquid chromatography coupled to a diode-array detector have been developed for the analysis of nalbuphine. The extraction step is the same for both techniques and involves extraction under alkaline conditions in chloroform-2-propanol-n-heptane (50:17:33, v/v/v) with levallorphan as the internal standard. After purification by acidic extraction and back alkaline extraction, drugs are derivatized with N,O-bis-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane for gas chromatography-mass spectrometry and directly injected for high-performance liquid chromatography-diode-array detection. The limits of detection are 2.0 and 25.0 ng/mg, respectively.
Keywords
Chromatography, High Pressure Liquid/methods, Gas Chromatography-Mass Spectrometry/methods, Humans, Nalbuphine/analysis, Nalbuphine/blood, Sensitivity and Specificity
Pubmed
Create date
18/10/2010 13:54
Last modification date
20/08/2019 16:10
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