Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells.

Details

Serval ID
serval:BIB_E6852BB7D1D6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells.
Journal
Blood
Author(s)
McKinney-Freeman S.L., Naveiras O., Yates F., Loewer S., Philitas M., Curran M., Park P.J., Daley G.Q.
ISSN
1528-0020 (Electronic)
ISSN-L
0006-4971
Publication state
Published
Issued date
09/07/2009
Peer-reviewed
Oui
Volume
114
Number
2
Pages
268-278
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Surface antigens on hematopoietic stem cells (HSCs) enable prospective isolation and characterization. Here, we compare the cell-surface phenotype of hematopoietic repopulating cells from murine yolk sac, aorta-gonad-mesonephros, placenta, fetal liver, and bone marrow with that of HSCs derived from the in vitro differentiation of murine embryonic stem cells (ESC-HSCs). Whereas c-Kit marks all HSC populations, CD41, CD45, CD34, and CD150 were developmentally regulated: the earliest embryonic HSCs express CD41 and CD34 and lack CD45 and CD150, whereas more mature HSCs lack CD41 and CD34 and express CD45 and CD150. ESC-HSCs express CD41 and CD150, lack CD34, and are heterogeneous for CD45. Finally, although CD48 was absent from all in vivo HSCs examined, ESC-HSCs were heterogeneous for the expression of this molecule. This unique phenotype signifies a developmentally immature population of cells with features of both primitive and mature HSC. The prospective fractionation of ESC-HSCs will facilitate studies of HSC maturation essential for normal functional engraftment in irradiated adults.
Keywords
Animals, Antigens, CD/analysis, Antigens, CD/metabolism, Cells, Cultured, Embryo, Mammalian/metabolism, Embryonic Stem Cells/metabolism, Female, Hematopoietic Stem Cells/metabolism, Mice, Mice, Inbred C57BL, Phenotype, Placenta/metabolism
Pubmed
Web of science
Create date
10/10/2022 23:05
Last modification date
11/10/2022 5:39
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