Characterization and Lytic Activity of Isolated Escherichia coli Bacteriophages against Escherichia coli in vitro.
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Version: Final published version
License: CC BY-ND 4.0
State: Public
Version: Final published version
License: CC BY-ND 4.0
Serval ID
serval:BIB_E641F8D5E361
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterization and Lytic Activity of Isolated Escherichia coli Bacteriophages against Escherichia coli in vitro.
Journal
Iranian journal of medical sciences
ISSN
0253-0716 (Print)
ISSN-L
0253-0716
Publication state
Published
Issued date
07/2020
Peer-reviewed
Oui
Volume
45
Number
4
Pages
298-303
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Escherichia coli (E. coli) is the most common cause of urinary tract infection (UTI) and typically treated with antibiotics. Unrestricted use of antibiotics may lead to the emergence of antibiotic-resistant bacteria. The present study aimed to isolate and characterize phages against E. coli from infected urine samples and to determine the lytic activity of phages against E. coliin vitro.
The present experimental study was conducted in the Laboratory of Bouali Sina Hospital (Sari, Iran) in May 2018. E. coli was identified from nine urine samples of patients with UTI using conventional microbiological methods. Bacteriophages were isolated from the infected urine specimens, and their lytic activity was determined using the spot test. The titer of the bacteriophages was measured using the double-layer agar technique. The morphology of the bacteriophages was revealed using transmission electron microscopy, and the latent time period and burst size were determined. Data were analyzed using the SPSS software package.
E. coli was isolated from nine infected urine samples. The lytic activity of bacteriophages against E. coli was determined using the spot test by observing the formation of inhibition zones. Transmission electron microscopy showed E. coli phages belonging to the Myoviridae family. The latent time period was 20 minutes with a burst size of 1,200 plaque-forming unit (PFU) per infected cell. The results of the double-layer agar assay showed that the titer of bacteriophages was 20×10 <sup>8</sup> PFU/mL.
The E. coli bacteriophage was isolated from infected urine samples and characterized, and their lytic activity against E. coli was determined in vitro.
The present experimental study was conducted in the Laboratory of Bouali Sina Hospital (Sari, Iran) in May 2018. E. coli was identified from nine urine samples of patients with UTI using conventional microbiological methods. Bacteriophages were isolated from the infected urine specimens, and their lytic activity was determined using the spot test. The titer of the bacteriophages was measured using the double-layer agar technique. The morphology of the bacteriophages was revealed using transmission electron microscopy, and the latent time period and burst size were determined. Data were analyzed using the SPSS software package.
E. coli was isolated from nine infected urine samples. The lytic activity of bacteriophages against E. coli was determined using the spot test by observing the formation of inhibition zones. Transmission electron microscopy showed E. coli phages belonging to the Myoviridae family. The latent time period was 20 minutes with a burst size of 1,200 plaque-forming unit (PFU) per infected cell. The results of the double-layer agar assay showed that the titer of bacteriophages was 20×10 <sup>8</sup> PFU/mL.
The E. coli bacteriophage was isolated from infected urine samples and characterized, and their lytic activity against E. coli was determined in vitro.
Keywords
Bacteriophages, Urinary tract infections, Escherichia coli, Bacteriophages, Urinary tract infections
Pubmed
Web of science
Create date
28/08/2020 8:54
Last modification date
30/04/2021 7:15