Regenerative Medicine at the Single Cell Level

Details

Serval ID
serval:BIB_E63C63F59CBF
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Institution
Title
Regenerative Medicine at the Single Cell Level
Title of the conference
9th Annual Congress of the French Society of Cell and Gene Therapy
Author(s)
Barrandon Yann, Lathion Stephanie
Address
Paris, France, June 13-15, 2010
ISBN
1043-0342
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
21
Series
Human Gene Therapy
Pages
767
Language
english
Notes
Meeting Abstract
Abstract
Adult stem cells are instrumental for renewal, regeneration, and repair. Self-renewal and the capacity to generate a tissue for an extended period of time (theoretically a life time) are fundamental properties of adult stem cells that allow longterm tissue reconstruction from a single stem cell as experimentally demonstrated with the bone marrow and the skin. Moreover, human epidermal stem cells (holoclones) can be extensively expanded and manipulated in culture before they are transplanted. We have taken advantage of these unique capacities to demonstrate the feasibility of a single epidermal stem cell approach for ex vivo gene therapy using recessive dystrophic epidermolysis bullosa (RDEB) as a model system. We have demonstrated that is possible to reconstruct a functional epidermis and anchoring fibers from the progeny of a single RDEB epidermal stem cell transduced with a Col7a1 cDNA by means of a SIN retrovirus. Demonstrations of safe proviral insertion, absence of tumorogenicity and of dissemination of the transduced engrafted cells meet regulatory affairs safety requirements.
Web of science
Create date
23/06/2010 13:01
Last modification date
20/08/2019 16:09
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