The conserved transcriptional regulator CdnL is required for metabolic homeostasis and morphogenesis in Caulobacter.

Details

Serval ID
serval:BIB_E5B9B0291A28
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The conserved transcriptional regulator CdnL is required for metabolic homeostasis and morphogenesis in Caulobacter.
Journal
PLoS genetics
Author(s)
Woldemeskel S.A., Daitch A.K., Alvarez L., Panis G., Zeinert R., Gonzalez D., Smith E., Collier J., Chien P., Cava F., Viollier P.H., Goley E.D.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Publication state
Published
Issued date
01/2020
Peer-reviewed
Oui
Volume
16
Number
1
Pages
e1008591
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Bacterial growth and division require regulated synthesis of the macromolecules used to expand and replicate components of the cell. Transcription of housekeeping genes required for metabolic homeostasis and cell proliferation is guided by the sigma factor σ70. The conserved CarD-like transcriptional regulator, CdnL, associates with promoter regions where σ70 localizes and stabilizes the open promoter complex. However, the contributions of CdnL to metabolic homeostasis and bacterial physiology are not well understood. Here, we show that Caulobacter crescentus cells lacking CdnL have severe morphological and growth defects. Specifically, ΔcdnL cells grow slowly in both rich and defined media, and are wider, more curved, and have shorter stalks than WT cells. These defects arise from transcriptional downregulation of most major classes of biosynthetic genes, leading to significant decreases in the levels of critical metabolites, including pyruvate, α-ketoglutarate, ATP, NAD+, UDP-N-acetyl-glucosamine, lipid II, and purine and pyrimidine precursors. Notably, we find that ΔcdnL cells are glutamate auxotrophs, and ΔcdnL is synthetic lethal with other genetic perturbations that limit glutamate synthesis and lipid II production. Our findings implicate CdnL as a direct and indirect regulator of genes required for metabolic homeostasis that impacts morphogenesis through availability of lipid II and other metabolites.
Keywords
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Caulobacter crescentus/genetics, Caulobacter crescentus/metabolism, Caulobacter crescentus/physiology, Cell Division, Conserved Sequence, Homeostasis, Metabolome, Transcription Factors/genetics, Transcription Factors/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
23/01/2020 16:10
Last modification date
26/04/2020 6:20
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